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Thromb Haemost 2002; 87(06): 1062-1068
DOI: 10.1055/s-0037-1613133
Review Article
Schattauer GmbH

Tissue Factor Activity Is Upregulated in Human Endothelial Cells Exposed to Oscillatory Shear Stress

Lucia Mazzolai
1   Division of Hypertension and Vascular Medicine, CHUV, Lausanne, Switzerland
,
Paolo Silacci
1   Division of Hypertension and Vascular Medicine, CHUV, Lausanne, Switzerland
,
Karima Bouzourene
1   Division of Hypertension and Vascular Medicine, CHUV, Lausanne, Switzerland
,
François Daniel
1   Division of Hypertension and Vascular Medicine, CHUV, Lausanne, Switzerland
,
Hans Brunner
1   Division of Hypertension and Vascular Medicine, CHUV, Lausanne, Switzerland
,
Daniel Hayoz
1   Division of Hypertension and Vascular Medicine, CHUV, Lausanne, Switzerland
› Author Affiliations
Further Information

Publication History

Received 19 November 2001

Accepted after revision 20 February 2002

Publication Date:
08 December 2017 (online)

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Summary

Hemodynamic forces play a critical role in the pathogenesis of atherosclerosis as evidenced by the focal nature of the disease. Oscillatory shear stress characterizes the hemodynamic environment of plaque-prone areas as opposed to unidirectional shear stress typical of plaque-free areas. These particular flow conditions modulate atherosclerosis-related genes. Tissue factor (TF) initiates blood coagulation, contributes to vascular remodeling, and is therefore a potential contributor in the development/progression of atherosclerosis. We investigated the effect of oscillatory and unidirectional flows on TF using an in vitro perfusion system. Human endothelial cells exposed for 24 h to oscillatory shear stress, significantly increased TF mRNA, and TF protein expression (1.5-and 1.75-fold, respectively, p <0.01), and surface TF activity (twofolds-increase). Expression of TF inhibitor (TFPI), mRNA and protein, remained unchanged as compared to static conditions. Conversely, cells exposed to unidirectional shear, showed a decrease in TF activity with a significant increase in TFPI mRNA and protein expression (1.5-and 1.8-fold, respectively, p <0.01). These results show for the first time that pulsatile oscillatory shear stress induces a procoagulant phenotype of endothelial cells which may favor formation/progression of atherothrombotic lesions.

Keywords

Tissue factor - tissue factor pathway inhibitor - shear stress - oscillatory flow - atherosclerosis
 

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