Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596856
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Good King-Henry (Chenopodium bonus-henricus L.) – a source of hepatoprotective flavonoids

Z Kokanova-Nedialkova
1   Department of Pharmacognosy, Faculty of Pharmacy, Medical University, Dunav str. 2, 1000, Sofia, Bulgaria
,
P Nedialkov
1   Department of Pharmacognosy, Faculty of Pharmacy, Medical University, Dunav str. 2, 1000, Sofia, Bulgaria
,
M Kondeva-Burdina
2   Laboratory of drug metabolism and drug toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Dunav str. 2, 1000, Sofia, Bulgaria
,
R Simenova
2   Laboratory of drug metabolism and drug toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Dunav str. 2, 1000, Sofia, Bulgaria
,
V Tsankova
2   Laboratory of drug metabolism and drug toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Dunav str. 2, 1000, Sofia, Bulgaria
,
S Nikolov
1   Department of Pharmacognosy, Faculty of Pharmacy, Medical University, Dunav str. 2, 1000, Sofia, Bulgaria
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

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Chenopodium bonus-henricus L. (Good King-Henry, wild spinach) is widespread in Europe, western Asia and parts of America. The plant is a substitube of spinach. The shoods and flower clusters are eaten like asparagus and broccoli [1]. In traditional medicine it is used as laxative, anthelmintic, antianemic and expectorant and for treatment of inflamated wounds [2 – 4]. Three new compounds 7 – 9 together with six known flavonoid glycosides 1 – 6 (Fig 1) of patuletin, spinacetin and 6-methoxykaempferol were indentified from the MeOH extract on the basis of spectroscopic methods (1D, 2D NMR, UV, IR, HRMS-ESI). The MeOH extract (60 µg/ml) of the aerial parts of C. bonus-henricus exerts hepatoprotective and antioxidant activities comparable to those of flavonoid complex silymarin in in vitro model of metabolic bioactivation, induced by tetrachloromethane. Along with decreased MDA quantity and increased level of GSH, seven days pre-treatment of rats with the MeOH extract (100 mg/kg/daily) also prevented the tetrachloromethane-caused oxidative damage by increasing antioxidant enzyme activities (CAT, SOD, GPx, GR and GST). All flavonoid glycosides (0.1 mM) significantly reduced the cellular damage caused by the hepatotoxic agent tetrachloromethane in rat hepatocytes, preserved cell viability and GSH level, decreased LDH leakage and thus reduced lipid peroxidation damage. Effects were similar to those of the positive control silybin. The cytotoxicity of isolated flavonoid glycosides, assessed in human hepatoma cell line HepG2 by using MTT-test, revealed a statistically significant cytotoxic effect only in high concentrations making flavonoid glycosides with 6-methoxykaempferol/spinacetin/patuletin skeleton a promising and save class of hepatoprotective compounds.

Zoom Image
Fig. 1: 6-Methoxyflavonol glycosides from the aerial parts of Chenopodium bonus-henricus L.

Acknowledgements: The study was supported by the Council of Medical Science of Medicinal University-Sofia (Grand 21/2014).

Keywords: Chenopodium bonus-henricus, Amaranthaceae, 6-methoxykaempferol, spinacetin, patuletin, anti-hepatotoxic activity.

References:

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