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DOI: 10.1055/s-0036-1596534
Antiprotozoal and heme-binding activity of 13 aromatic and medicinal plants from Ahaggar, Algeria
Publication History
Publication Date:
14 December 2016 (online)
Thirteen species from Ahaggar area were selected for antiprotozoal screening on the basis of ethnopharmacological uses and/or chemotaxonomy. Among the selected plants 10 Asteraceae, 2 Apiaceae and 1 Lamiaceae were collected in the Ahaggar National Park, in the Southern Sahara [1, 2]. Both antiplasmodial activity (P. falciparum, chloroquino-resistant FcB1/Colombia) and antileishmanial activity (promastigotes from L. amazonensis) were evaluated. 7 species exhibited a significant antileshmanial activity (IC50 < 10 µg/ml) and 8 species showed significant antiplasmodial activity (IC50 ≤1 µg/l), but no correlation between both activities was observed. As antiplasmodial activity could be associated with inhibition of heme degradation into hemozoin, with subsequent Plasmodium death, a combined heme-binding assay was carried out in order to identify stable heme-adducts [3]. Comparison of LC-MS spectra of crude extracts with or without heme allowed us to identify m/z values of compounds involved in the heme-adduct formation. Among all identified adducts, we found that two methoxyflavones isolated from P. monodiana can form adducts with heme.
Acknowledgements: Authors thanks the ChemBioFight European funding (FP7-people-2010-IRSES), Algerian government for PROFAS training financial support and Director of Ahaggar National Park.
Keywords: Ethnopharmacology, antiprotozoal, heme-binding, Ahaggar.
References:
[1] Ozenda P. Flore et végétation du Sahara, Ed. CNRS, 2004: 438
[2] Hammiche V, Maiza K. Traditional medicine in Central Sahara: Pharmacopoeia of Tassili N'ajjer. J Ethnopharmacol 2006; 105: 358 – 367
[3] Muñoz-Durango K, Maciuk A, Harfouche A, Torijano-Gutiérrez S, Jullian JC, Quintin J, Spelman K, Mouray E, Grellier P, Figadère B. Detection, characterization, and screening of heme-binding molecules by mass spectrometry for malaria drug discovery. Anal Chem 2012; 84: 3324 – 3329
Plant |
Fraction |
Antileishmania activity (IC50 µg/ml) |
Antiplasmodial activity (IC50 µg/ml) |
m/z values of compounds forming heme-adducts |
Ammodaucus leucotrichus (fruit), Apiaceae |
EtOAc |
6.5 |
> 100 |
389 [M+H]+ |
MeOH |
- |
79.3 ± 7.7 |
381 [M+H]+ |
|
Anvillea radiata (aerial part), Asteraceae |
EtOAc |
10.2 |
2.31 ± 0.86 |
277 [M+H]+, 287 [M+H]+, 344 [M+H]+ |
MeOH |
- |
> 100 |
235 [M+H]+ |
|
Artemisia campestris ssp glutinosa (aerial part), Asteraceae |
EtOAc |
17.26 |
1.21 ± 0.16 |
315 [M+H]+, 330 [M+H]+ , 345 [M+H]+, 359 [M+Na]+ |
MeOH |
- |
0.72 ± 0.27 |
355 [M+H]+ |
|
Artemisia judaica ssp saharensis (aerial part), Asteraceae |
EtOAc |
8.93 |
1.13 ± 0.19 |
314 [M+H]+, 344 [M+H]+, 420 [M+H]+ |
MeOH |
- |
> 100 |
- |
|
Cotula anthemoides (whole plant), Asteraceae |
EtOAc |
6.59 |
78.4 ± 15.6 |
250 [M+H]+, 264 [M+H]+ |
MeOH |
- |
1.20 ± 0.32 |
250 [M+H]+, 395 [M+H]+ |
|
Deverra scoparia (aerial part)= Pituranthos scoparia, Apiaceae |
EtOAc |
32.54 |
4.60 ± 1.50 |
239 [M+H]+, 357 [M+Na]+, 451 [M+Na]+ |
MeOH |
- |
20.4 ± 2.1 |
395 [M+Na]+ |
|
Deverra scoparia (root) = Pituranthos scoparia, Apiaceae |
EtOAc |
7.27 |
> 100 |
301 [M+H]+, 451 [M+H]+ |
MeOH |
- |
0.99 ± 0.41 |
365 [M+H]+, 381 [M+H]+ |
|
Nauplius graveolens (aerail parts)= Asteriscus graveolens, Asteraceae |
EtOAc |
6.95 |
- |
- |
MeOH |
- |
> 100 |
235 [M+H]+ |
|
Pentzia monodiana (aerial parts), Asteraceae |
EtOAc |
13.63 |
3.53 ± 1.08 |
359 [M+H]+, 389 [M+H]+ |
MeOH |
- |
> 100 |
- |
|
Pulicaria crispa (aerial parts) |
EtOAc |
14.93 |
36.2 ± 4.1 |
347 [M+H]+, 533 [M+H]+, 549 [M+H]+ |
MeOH |
- |
8.80 ± 1.80 |
156 [M+H]+, 235 [M+H]+ |
|
Pulicaria incisa (aerial parts) |
EtOAc |
8.37 |
4.57 ± 1.23 |
329 [M+H]+, 353 [M+H]+ |
MeOH |
- |
34.2 ± 8.1 |
- |
|
Rhaponticum acaule (root) |
EtOAc |
14 |
4.7 ± 0.46 |
435 [M+H]+ |
MeOH |
- |
> 100 |
365 [M+H]+ |
|
Rhaponticum acaule (aerial parts) |
EtOAc |
26 |
1.27 ± 0.49 |
385 [M+H]+, 445 [M+H]+, 585 [M+H]+ |
MeOH |
- |
40.2 ± 4.93 |
434 [M+H]+ |
|
Salvia chudaei (leaves), Lamiaceae |
EtOAc |
- |
> 100 |
329 [M+H]+, 369 [M+H]+ |
MeOH |
- |
64.2 ± 40.5 |
399 [M+H]+ |