Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596524
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

In vitro and cellular antioxidant activities of Antidesma thwaitesianum Müll. Arg. (Euphorbiaceae) leaf extracts

P Hansakul
1   Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand
3   Center of Excellence in Applied Thai Traditional Medicine Research, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand
,
B Dechayont
2   Department of Applied Thai Traditional, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand
3   Center of Excellence in Applied Thai Traditional Medicine Research, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand
,
N Nuengchamnong
4   Science Laboratory Centre, Faculty of Science, Naresuan University, Phitsanulok, 65000, Thailand
,
A Itharat
2   Department of Applied Thai Traditional, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand
3   Center of Excellence in Applied Thai Traditional Medicine Research, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

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Fresh and fruit waste extracts of Antidesma thwaitesianum Müll. Arg. (Euphorbiaceae) possessed potent in vitro and cellular antioxidant activities [1]. Its leaves are edible as a fresh vegetable, and its leaf decoction is used to treat degenerative diseases in Thai traditional medicine. However, no scientific evidence supporting its health benefits has been reported. Accordingly, we aimed to determine in vitro and cellular antioxidant activities of A. thwaitesianum leaf extracts obtained from different extraction methods. The fresh leaf extract was prepared by blending. Additionally, the dried leaf extracts were obtained by decoction and maceration, including the extract obtained by decocting the residue left after maceration. In chemical assays, all of the tested leaf extracts exerted scavenging activity against DPPH and ABTS radicals (EC50 = 3.54 – 7.46 µg/mL; 6.13 – 11.81 µg/mL). The maceration and decoction extracts possessed the highest antioxidant activity, similar to the standard, Trolox. In cell-based assays, only the ethanolic extract exerted the most potent superoxide (O2 .-) and nitric oxide (NO.) scavenging activity (EC50 = 58.12; 71.90 µg/mL). In contrast, the decoction extract possessed only strong NO. scavenging action (EC50 = 91.20 µg/mL). The decoction and maceration extracts contained high total phenolic and flavonoid contents, which were partially correlated with their cellular antioxidant activity. Due to its highest antioxidant potential, the first comprehensive identification of active components of A. thwaitesianum ethanolic extract was performed by HPLC-ESI-QTOF-MS. In the analysis, 145 compounds were obtained, as well as 14 flavonoids and 35 phenolic acids were tentatively identified. This is the first report describing in vitro and cellular antioxidant effects of leaf extracts of A. thwaitesianum, supporting the traditional use of its leaf decoction. Moreover, its ethanolic leaf extract has a great potential as dietary supplements.

Acknowledgements: This study was financially supported by National Research University Project of Thailand, Office of the Higher Education Commission, the National Research Council of Thailand (NRCT), and Faculty of Medicine, Thammasat University.

Keywords: Antidesma thwaitesianum Müll. Arg., cellular antioxidant activity, cell-based assays.

References:

[1] Hansakul P, Dechayont, B Phuaklee P, Prajuabjinda O, Juckmeta T, Itharat A. Cytotoxic and antioxidant activities of Antidesma thwaitesianum Müll Arg (Euphorbiaceae) fruit and fruit waste extracts. Trop J Pharm Res, 2015; 14: 627 – 634