Effect of antioxidant activity of Pyrola japonica on H2O2-induced oxidative stress in HSEC cells

Oxidative stress contributes many pathological conditions and disease. However, antioxidant systems are usually effective in blocking oxidative stress. The purpose of this study was to determine the antioxidant activity of Pyrola japonica Klenze ex Alef. [Ericaceae] (TKM2082-W) water extract in H₂O₂-treated HSEC cells. Total phenolic and flavonoid contents of TKM2082-W were 59.6 mg/g and 26.1 mg/g respectively. The TKM2082-W was screened for chemical antioxidant activity by radical scavenging such as DPPH scavenging, superoxide anion radical scavenging and ABTS+ scavenging assay at different concentration. Throughout the studies TKM2082-W showed marked antioxidant activity. The pretreatment TKM2082-W also decreases intracellular reactive oxygen species (ROS) levels in H₂O₂-treated HSEC cells. The results of the present study concluded that the TKM2082-W is a source of natural antioxidants which might be helpful in preventing the progress of various oxidative stress mediated diseases including aging.

Fig. 1: Effect of TKM2082-W on hydrogen dioxide (H₂O₂)-mediated ROS induction in HSEC cells. H9C2 were pre-treated with DCFH-DA for 30 min, followed by the indicated concentration of TKM2082-W and 1 mM of H₂O₂ treatment for 3h incubation. Levels of intracellular ROS were measured by flow cytometry. (A) Unstaining, (B) Control, (C) H₂O₂-treated cells, TKM2082-W (D) 62.5 ug/ml and H₂O₂-treated cells (E) 125 ug/ml and H₂O₂-treated cells (F) 250 ug/ml and H₂O₂-treated cells.

Acknowledgements: This work was supported by Ministry of Health & Welfare “The Traditional Korean Medicine Industry Development Product R&D Program (C100001)”.

Keywords: Antioxidant, Free radical scavenging activity, ROS.

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