Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596143
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Chemical profiling of Actaea species and commercial products using UPLC-QTof-MS

MHM Sharaf
1   American Herbal Products Association, 8630 Fenton Street, Silver Spring, MD 20910, USA
,
J Yuk
2   Waters Corporations, 34 Maple Street, Milford, MA 01757, USA
,
K Yu
2   Waters Corporations, 34 Maple Street, Milford, MA 01757, USA
,
M Wrona
2   Waters Corporations, 34 Maple Street, Milford, MA 01757, USA
,
G Isaac
2   Waters Corporations, 34 Maple Street, Milford, MA 01757, USA
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Publikationsdatum:
14. Dezember 2016 (online)

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Black cohosh (Actaea racemosa L.) is a native North American species, where it is widely used as an herbal supplement for the relief of menopausal symptoms. It is available in Europe as a phytomedicine since the 1950 s. Black cohosh commercial products were ranked in the top ten of supplement sales in the USA [1]. In addition to A. racemosa, there are other North American and Asian Actaea species. Due to the continual increase in sales and collection of A. racemosa, mix up and adulteration with related species could be seen in the market.

Morphological examination to genetic testing has been commonly used to authenticate different Actaea species. Studies using instruments such as HPTLC, HPLC-DAD, HPLC-ELSD, GC-MS, NMR, and LC-MS have also been used to profile these species. Many of the studies have focused on identifying key chemical components [2]. A newer approach has been the development of untargeted methods to compare complete chemical profile using authenticated references.

In the present study, a multivariate statistical method using UPLC-QTof-MS was used for the classification of different Actaea species and commercial black cohosh products. Principal component analysis allowed a holistic approach in showing distinct clustering between the different species and black cohosh commercial products. Differential analysis of results across several Actaea species was performed which facilitated the identification and quantitation of potential metabolite markers. Significant markers that differentiate between these species and for black cohosh commercial products were identified. The identification of the markers was based on exact mass precursor ion, theoretical isotopic distribution, and high energy fragment ion information. Theoretical fragmentation of the candidate compounds was also performed to increase the confidence of their identification. We distinguished useful marker compounds like cimifugin derivatives, triterpene glycosides and alkaloids that differentiate between Actaea species.

Keywords: Black cohosh, Actaea racemosa L., authentication, UPLC-QTof-MS.

References:

[1] Smitha T, Lynch ME, Johnson J, Kawa K, Bauman H, Blumenthal M. Herbal dietary supplement sales in US increase 6.8% in 2014. HerbalGram 2015; 107: 52 – 59

[2] Jiang B, Ma C, Motley T, Kronenberg F, Kennelly EJ. Phytochemical fingerprinting to thwart black cohosh adulteration: a 15 Actaea species analysis. Phytochem Anal 2011; 22: 339 – 351