Anti Tumoral Properties Of Centipede Scolopendra Extract On Human Lung Cancer Cells In Vitro And In Vivo

Objective: To investigate efficiency of Centipede Scolopendra Extract (CSE) on apoptosis induction, proliferation inhibition to Human A549 cell line and growth suppression of subcutaneous transplanted sarcoma in nude mice. Methods: CSE was prepared by enzymolysis and acetone precipitation methods and used to treat human lung cancer A549 cell line. Proliferation inhibition was evaluated by MTT assay and calculate influence of half inhibit concentration (IC₅₀). Cell morphological change and apoptosis were detected by flow cytometry and Hoechst stain. The subcutaneous transplanted sarcoma model was prepared with nude mice and suppression of tumor growth was evaluated compared with cisplatin (CDDP). Results: After 48h CSE treatment, proliferation of A549 cells were inhibited with dose-dependent and IC₅₀ value was 0.603 mg/mL. The G₀/G₁ phase of cells was down regulated and G₂/M, S phase cells were up regulated. The apoptotic character cells have been found by Hoechst stain. In vivo experiment, the tumor weight and volume were decreased significantly compared with model control group (P< 0.01). Conclusion: The CSE can inhibit proliferation and induce apoptosis through arresting A549 cells at G₂/M phase and suppress growth of subcutaneous transplanted sarcoma of lung cancer in nude mice.