The Influence Of Phosphate And Nitrate On Growth And Chemical Diversity Produced By The Freshwater Cyanobacterium Nostoc Sp. (UIC 10110)

CM Crnkovic; D May; J Orjala

doi:10.1055/s-0036-1578660

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Planta Med 2016; 82 - PB12
DOI: 10.1055/s-0036-1578660

The Influence Of Phosphate And Nitrate On Growth And Chemical Diversity Produced By The Freshwater Cyanobacterium Nostoc Sp. (UIC 10110)

CM Crnkovic ¹^,², D May ¹, J Orjala ¹

¹Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA
²CAPES Foundation, Ministry of Education of Brazil, Brasília – DF 70040 – 020, Brazil

Congress Abstract

Growth conditions play an important role in the production of secondary metabolites by cultured cyanobacteria. We investigated the influence of two concentrations of phosphate and nitrate on growth and chemical diversity produced by the freshwater cyanobacterium Nostoc sp. (UIC 10110). Biological triplicates were cultured in four different media following a 2²-factorial design. Metabolomic profiling by mass spectrometry and analysis using XCMS Online allowed detection of secondary metabolites and relative quantification. Nitrate concentrations drastically influenced cell growth, while phosphate levels induced significant differences in the relative quantification of merocyclophanes produced by the strain. This method also revealed a potential novel compound not identified by previous studies of this strain.