HPLC Profiling Of Phyllanthus Emblica L. Extracts

Phyllanthus emblica L. has a significant position in Ayurveda medicine. It is extensively used in herbal preparations and as a food supplement [1]. This study aimed at developing an HPLC method for quantitative determination of eight compounds that were previously isolated from P. emblica L. (gallic acid, 1-O-galloyl glycerol, 1,2,4,6-tetra-O-galloyl-β-D-glucopyranoside, kaempferol, quercetin, kaempferol-3-O-α-L-rhamnose, quercetin-3-O-α-L-rhamnose and quercetin-3-O-β-D-glucopyranoside) in the methanolic extracts prepared from the leaves, fruits, pericarp of the fruits, seeds, stem branches and stem bark of P. emblica L. Compounds identification based on comparing on-line chromatograms and UV spectra with those of the standard compounds. The results showed that gallic acid is the major component in the methanolic extract of the fruits and pericarp of the fruits (143.82, 211.55 mg/g respectively) [2]. 1, 2, 4, 6-tetra-O-galloyl-β-D-glucopyranoside was the major component in leaves (25.6 mg/g), kaempferol and quercetin were detected in leaves and fruits only. Concentrations of the other compounds were detected in the all prepared extracts.

References:[1] Bhandari, P.R. and KamDOD, M.A. (2012). Int J Green Pharm, 6(4): 257 – 269. [2] Sawant, L.P. (2010). J Anal & Bioanal Tech, 1(3):2 – 4