Simultaneous Analysis Of Aegeline And Six Coumarins From Plant Aegle Marmelos Using UHPLC-PDA-MS And Aegeline Chiral Separation Using LC-TOf-MS

A fast UHPLC-PDA method was developed for the simultaneous analysis of one alkaloid, aegeline, and six coumarins [namely, umbelliferone, scopoletin, marmesinin, 8-hydroxypsoralen, angelicin and marmelosin] from leaf, fruit, root and bark of Aegle marmelos (L.) Corrêa (Rutaceae). The method was validated for linearity, accuracy, repeatability, limits of detection (LOD) and limits of quantification (LOQ). The linearity range (r ²> 0.99) of the seven compounds was found to be 0.5 – 250 µg/mL, LOD and LOQ for seven compounds were found to be 0.1 and 0.5 µg/mL, respectively. The developed UHPLC method is simple, fast and especially suitable for quality control analysis of coumarins and aegeline from A. marmelos and commercial dietary supplements. Mass spectrometry was used for the confirmation of coumarins and aegeline from different plant parts and dietary supplements. In addition, a novel chiral LC-ToF-MS method was developed for enantiomeric separation of aegeline. By applying this method, distribution of enantiomers of aegeline from different parts of A. marmelos and aegeline-containing dietary supplements are reported for the first time.

Acknowledgements: This research is supported in part by “Science Based Authentication of Dietary Supplements” funded by the Food and Drug Administration grant number 1U01FD004246 – 05, the United States Department of Agriculture, Agricultural Research Service, Specific Cooperative Agreement No. 58 – 6408 – 1-603 – 07.