PDF herunterladen
J Reconstr Microsurg 2016; 32(05): 395-401
DOI: 10.1055/s-0036-1571348
Original Article: WSRM 2015 Scientific Paper
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Effects of Bezafibrate on the Survival of Random Skin Flaps in Rats

Bin Lin
1   Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical University of Wenzhou Medical University, Wenzhou, China
2   Department of Pharmacy, Changxing People's Hospital, Changxing, China
,
Yuting Lin
1   Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical University of Wenzhou Medical University, Wenzhou, China
3   Department of Hand and Plastic Surgery, The Renji College of Wenzhou Medical University, Wenzhou, China
,
Dingsheng Lin
1   Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical University of Wenzhou Medical University, Wenzhou, China
,
Bin Cao
1   Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical University of Wenzhou Medical University, Wenzhou, China
› Institutsangaben
Weitere Informationen

Publikationsverlauf

27. Juli 2015

17. Dezember 2015

Publikationsdatum:
12. Februar 2016 (online)

Auch verfügbar auf
    Lizenzen und Reprints

Abstract

Background Bezafibrate is widely used in clinics for its comparable angiogenic effect. Our research is to investigate the effect of bezafibrate on random skin flap survival.

Materials and Methods The “McFarlane flap” rat models were established in 30 male Sprague-Dawley rats which were divided into two groups. The treatment group was given bezafibrate (400 mg/kg/day; gavage administration), and the control group received the vehicle. The flap surviving area was measured after 7 days, and the tissue samples were taken for histological analysis and edema measurement. Vascular endothelial growth factor (VEGF) was determined using immunohistochemical methods. Superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were examined with kits.

Results Seven days after the operation, the surviving area in the treatment group was larger than in the control group (p < 0.01). The expression of VEGF was increased in the treatment group compared with that in the control group. And the activity of SOD was lower in the treatment group compared with those in the control group (p < 0.01). However, tissue edema, inflammatory cell infiltration, and MDA level were markedly lower in the treatment group than those in the control group (p < 0.01).

Conclusion Bezafibrate improves the survival of random skin flaps effectively.

Keywords

bezafibrate - random skin flap - survival

Note

Bin Lin and Yuting Lin contributed equally to this work.


 

  • References

  • 1 Cai L, Huang W, Lin D. Effects of traditional Chinese medicine Shuxuetong injection on random skin flap survival in rats. ScientificWorldJournal 2014; 2014: 816545
    PubMedGoogle Scholar
  • 2 Yang M, Sheng L, Li H, Weng R, Li QF. Improvement of the skin flap survival with the bone marrow-derived mononuclear cells transplantation in a rat model. Microsurgery 2010; 30 (4) 275-281
    PubMedGoogle Scholar
  • 3 Im MJ, Manson PN, Bulkley GB, Hoopes JE. Effects of superoxide dismutase and allopurinol on the survival of acute island skin flaps. Ann Surg 1985; 201 (3) 357-359
    CrossrefPubMedGoogle Scholar
  • 4 Shalom A, Friedman T, Westreich M. Effect of aspirin and heparin on random skin flap survival in rats. Dermatol Surg 2008; 34 (6) 785-790 , discussion 790
    PubMedGoogle Scholar
  • 5 Gideroglu K, Alagoz S, Uygur F, Evinc R, Celikoz B, Bugdayci G. Effects of nebivolol on skin flap survival: a randomized experimental study in rats. Curr Ther Res Clin Exp 2008; 69 (5) 449-458
    CrossrefPubMedGoogle Scholar
  • 6 Chintalgattu V, Harris GS, Akula SM, Katwa LC. PPAR-gamma agonists induce the expression of VEGF and its receptors in cultured cardiac myofibroblasts. Cardiovasc Res 2007; 74 (1) 140-150
    CrossrefPubMedGoogle Scholar
  • 7 Wang Y, Wang Y, Yang Q , et al. Effects of bezafibrate on the expression of endothelial nitric oxide synthase gene and its mechanisms in cultured bovine endothelial cells. Atherosclerosis 2006; 187 (2) 265-273
    CrossrefPubMedGoogle Scholar
  • 8 Duez H, Fruchart JC, Staels B. PPARS in inflammation, atherosclerosis and thrombosis. J Cardiovasc Risk 2001; 8 (4) 187-194
    CrossrefPubMedGoogle Scholar
  • 9 Abdelrahman M, Sivarajah A, Thiemermann C. Beneficial effects of PPAR-gamma ligands in ischemia-reperfusion injury, inflammation and shock. Cardiovasc Res 2005; 65 (4) 772-781
    CrossrefPubMedGoogle Scholar
  • 10 Kelly CP, Gupta A, Keskin M, Jackson IT. A new design of a dorsal flap in the rat to study skin necrosis and its prevention. J Plast Reconstr Aesthet Surg 2010; 63 (9) 1553-1556
    CrossrefPubMedGoogle Scholar
  • 11 Mandriota SJ, Pyke C, Di Sanza C, Quinodoz P, Pittet B, Pepper MS. Hypoxia-inducible angiopoietin-2 expression is mimicked by iodonium compounds and occurs in the rat brain and skin in response to systemic hypoxia and tissue ischemia. Am J Pathol 2000; 156 (6) 2077-2089
    CrossrefPubMedGoogle Scholar
  • 12 Khazaei M, Salehi E, Rashidi B. Pan-PPAR agonist, bezafibrate, restores angiogenesis in hindlimb ischemia in normal and diabetic rats. Int J Pept 2012; 2012: 637212
    PubMedGoogle Scholar
  • 13 Ozkan O, Ozgentas HE. Combination of rat vest, teeth shortening, and nail cutting to prevent autocannibalization and protect surgical flaps. Plast Reconstr Surg 2006; 117 (5) 1671
    CrossrefPubMedGoogle Scholar
  • 14 Chen Z, Wang T, Cai L , et al. Clinicopathological significance of non-small cell lung cancer with high prevalence of Oct-4 tumor cells. J Exp Clin Cancer Res 2012; 31: 10
    CrossrefPubMedGoogle Scholar
  • 15 Kenne E, Erlandsson A, Lindbom L, Hillered L, Clausen F. Neutrophil depletion reduces edema formation and tissue loss following traumatic brain injury in mice. J Neuroinflammation 2012; 9: 17
    CrossrefPubMedGoogle Scholar
  • 16 Ozkan F, Senayli Y, Ozyurt H, Erkorkmaz U, Bostan B. Antioxidant effects of propofol on tourniquet-induced ischemia-reperfusion injury: an experimental study. J Surg Res 2012; 176 (2) 601-607
    CrossrefPubMedGoogle Scholar
  • 17 Parlakpinar H, Ozer MK, Sahna E, Vardi N, Cigremis Y, Acet A. Amikacin-induced acute renal injury in rats: protective role of melatonin. J Pineal Res 2003; 35 (2) 85-90
    CrossrefPubMedGoogle Scholar
  • 18 Kailiang Z, Yihui Z, Dingsheng L, Xianyao T. Effects of muscone on random skin flap survival in rats. J Reconstr Microsurg 2015; (e-pub ahead of print)
    PubMedGoogle Scholar
  • 19 Lin Y, Lin B, Lin D, Huang G, Cao B. Effect of thymosin β4 on the survival of random skin flaps in rats. J Reconstr Microsurg 2015; 31 (6) 464-470
    Artikel in Thieme ConnectPubMedGoogle Scholar
  • 20 Changizi Ashtiyani S, Zohrabi M, Hassanpoor A, Hosseini N, Hajihashemi S. Oral administration of the aqueous extract of Rosmarinus officinalis in rats before renal reperfusion injury. Iran J Kidney Dis 2013; 7 (5) 367-375
    PubMedGoogle Scholar
  • 21 Zhang F, Oswald T, Lin S , et al. Vascular endothelial growth factor (VEGF) expression and the effect of exogenous VEGF on survival of a random flap in the rat. Br J Plast Surg 2003; 56 (7) 653-659
    CrossrefPubMedGoogle Scholar
  • 22 Fang T, Lineaweaver WC, Chen MB, Kisner C, Zhang F. Effects of vascular endothelial growth factor on survival of surgical flaps: a review of experimental studies. J Reconstr Microsurg 2014; 30 (1) 1-13
    Artikel in Thieme ConnectPubMedGoogle Scholar
  • 23 Lineaweaver WC, Lei MP, Mustain W, Oswald TM, Cui D, Zhang F. Vascular endothelium growth factor, surgical delay, and skin flap survival. Ann Surg 2004; 239 (6) 866-873 , discussion 873–875
    CrossrefPubMedGoogle Scholar
  • 24 Xiao-Xiao T, Sen-Min W, Ding-Sheng L. Effects of vinpocetine on random skin flap survival in rats. J Reconstr Microsurg 2013; 29 (6) 393-398
    Artikel in Thieme ConnectPubMedGoogle Scholar
  • 25 Cui J, Liu L, Zou J , et al. Protective effect of endogenous hydrogen sulfide against oxidative stress in gastric ischemia-reperfusion injury. Exp Ther Med 2013; 5 (3) 689-694
    PubMedGoogle Scholar
  • 26 Bulhak AA, Sjöquist PO, Xu CB, Edvinsson L, Pernow J. Protection against myocardial ischaemia/reperfusion injury by PPAR-α activation is related to production of nitric oxide and endothelin-1. Basic Res Cardiol 2006; 101 (3) 244-252
    CrossrefPubMedGoogle Scholar
  • 27 von Knethen A, Brüne B. PPARgamma—an important regulator of monocyte/macrophage function. Arch Immunol Ther Exp (Warsz) 2003; 51 (4) 219-226
    PubMedGoogle Scholar