Planta Med 2015; 81 - PW_167
DOI: 10.1055/s-0035-1565791

Effect of Calliandra portoricensis extract on the pharmacokinetics of glibenclamide in rats

GE Ukpo 1, 2, SO Ogbonnia 1, 2, TS Ehianeta 1, 2, WA Badiru 1, 2
  • 1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Lagos, Lagos, Nigeria
  • 2Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Lagos, Nigeria

Calliandra portoricensis (Jacq.) Benth (Fam. Leguminosae), is a West African plant used traditionally for its medicinal values e.g. as analgesic and anti-inflammatory. It is widely used with orthodox medicines such as glibenclamide, a potent oral sulfonylurea antidiabetic agent. This study aims at evaluating the disposition of the pharmacokinetics of glibenclamide with or without C. portoricensis (CP) in rats.

Twenty rats of both sexes weighing 150 ± 15 g and allowed to acclimatize for 2 weeks were used for the study. All the animals received a single oral dose of 0.6 mg/kg body weight of glibenclamide, and blood samples (0.1 mL) were withdrawn from each rat by retro-orbital puncture at 0, 0.5, 1, 2, 3, 4, 6, 8, 12, 24 and 48 hrs to determine the level of glibenclamide in the plasma at each time using a validated HPLC method with a UV Detector at λ= 253nm. After 2 weeks they received 500 mg/kg of CP orally for 5 consecutive days and on the 5th day were treated with the same dose of glibenclamide orally 15 minutes post administration of CP and the level of glibenclamide in the plasma determined as earlier stated.

No significant (p ≥0.05) change in any pharmacokinetic parameter (elimination half-life, t½ and Cmax, (Tmax), volume of distribution (Vd) and clearance (CL)) was observed compared to the control. The non-significant change observed in Cmax and Tmax could be an indication of reduced rate of drug absorption which was demonstrated by the reduction in AUC. Drug elimination found to be slower after co-administration of glibenclamide; CP non-significantly decreased t½ but significantly increased Vd.