Evaluation of sulphated polysaccharide-protein complexes isolated from marine algae on colon carcinoma by MTT

AA Matloub; K Mahmoud

doi:10.1055/s-0035-1565581

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Planta Med 2015; 81 - PM_204
DOI: 10.1055/s-0035-1565581

Evaluation of sulphated polysaccharide-protein complexes isolated from marine algae on colon carcinoma by MTT

AA Matloub ¹, K Mahmoud ¹

¹Pharmacognosy Department, National Research Centre, 33 Bohouth Street, Dokki, Cairo, Egypt., Cairo, Egypt

Congress Abstract

Cold and hot extraction methods (CEM and HEM) were used for polysaccharides from Dictyopetris membraceae, Padina pavonia, Colpomenia sinusa, Ulva fasciata, Enteromorpha intestinalis, Corallina officinalis, Petrocladia capillraceae, Jania rubes and Spirulina platensis. These polysaccharides were tested against a colon carcinoma cell line (HCT115) at various concentrations (100 – 6.26 µg/mL) using the MTT assay.

The polysaccharides were characterized as sulphated polysaccharide-protein complexes. The hot polysaccharide extract of J. rubens and D. membranacea exhibited the most potent cytotocicity against HCT115 with an IC₅₀ of 24.7 and 38.2 µg/mL, respectively. The other polysaccharides showed various cytotoxic activity against HCT115 with an IC₅₀ ranging from 60.8 – 79.8 µg/mL. Therefore the sulphated polysaccharides isolated from macroalgae might become important sources for drug development for treatment of colon carcinoma.