Download PDF
Facial Plast Surg 2014; 30(06): 670-675
DOI: 10.1055/s-0034-1396527
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

PRFM Enhance Wound Healing Process in Skin Graft

Mirta Reksodiputro
1   Department of ENT, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
,
Dini Widodo
1   Department of ENT, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
,
Jenny Bashiruddin
1   Department of ENT, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
,
Nurjati Siregar
2   Department of Pathology Anatomy, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
,
Safarina Malik
3   Department of Biology Molecular, Eijkman Institute, Jakarta, Indonesia
› Author Affiliations
Further Information

Publication History

Publication Date:
23 December 2014 (online)

Also available at
    Permissions and Reprints

Abstract

Facial plastic and reconstructive surgery often used skin graft on defects that cannot be covered primarily by a local flap. However, wound healing using skin graft is slow, most of the time the graft is contractured and the take of graft is not optimal. Platelet rich fibrin matrix (PRFM) is a new generation of concentrated platelets that produce natural fibrin and reported to speed up the healing process. Application of PRFM in the skin graft implants is expected to increase the survival of the graft. We used porcine as animal models to elucidate the effect of autologous PRFM on wound healing in full-thickness (FTSG) and split-thickness (STSG) skin grafts. Survival level of the skin graft was determined by using ImageJ software based on the formation of collagen type 1 and graft take. We observed that the use of PRFM in FTSG and STSG increased type 1 collagen formation. We also found that PRFM addition in STSG gave the best skin graft take.

Keywords

PRFM - skin graft - wound healing - porcine
 

  • References

  • 1 Hom DB. New developments in wound healing relevant to facial plastic surgery. Arch Facial Plast Surg 2008; 10 (6) 402-406
    CrossrefPubMedGoogle Scholar
  • 2 Metcalfe AD, Ferguson MWJ. Tissue engineering of replacement skin: the crossroads of biomaterials, wound healing, embryonic development, stem cells and regeneration. J R Soc Interface 2007; 4 (14) 413-437
    CrossrefPubMedGoogle Scholar
  • 3 Everts PAM, Knape JTA, Weibrich G , et al. Platelet-rich plasma and platelet gel: a review. J Extra Corpor Technol 2006; 38 (2) 174-187
    CrossrefPubMedGoogle Scholar
  • 4 Sclafani AP. Safety, efficacy, and utility of platelet-rich fibrin matrix in facial plastic surgery. Arch Facial Plast Surg 2011; 13 (4) 247-251
    CrossrefPubMedGoogle Scholar
  • 5 Sclafani AP. Applications of platelet-rich fibrin matrix in facial plastic surgery. Facial Plast Surg 2009; 25 (4) 270-276
    Article in Thieme ConnectPubMedGoogle Scholar
  • 6 Lucarelli E, Beretta R, Dozza B , et al. A recently developed bifacial platelet-rich fibrin matrix. Eur Cell Mater 2010; 20: 13-23
    PubMedGoogle Scholar
  • 7 Hom DB, Tope WD. Minimal invasive options and skin grafts for cutaneous reconstruction. In: Papel ID, Frodel JL, Holt GR, Larrabee WF, Nachlas N, Park SS, , et al, eds. Facial Plastic and Reconstructive Surgery. New York, NY: Thieme; 2008: 703-719
    Google Scholar
  • 8 Paletta CE, Pokorny JJ, Rumbolo P. Skin grafts. In: Mathes SJ, Hentz VR, , eds. Plastic surgery. Vol. 1. Philadelphia, PA: Saunders Elsevier; 2006: 293-316
    Google Scholar
  • 9 Scherer SS, Pietramaggiori G, Orgill DP. Skin graft. In: Neligan PC, Gurtner GC, , eds. Plastic Surgery-Principles. 3rd ed. Vol. 1. Seattle, WA: Elsevier; 2014: 319-338
    Google Scholar
  • 10 O'Connell SM, Impeduglia T, Hessler K, Wang XJ, Carroll RJ, Dardik H. Autologous platelet-rich fibrin matrix as cell therapy in the healing of chronic lower-extremity ulcers. Wound Repair Regen 2008; 16 (6) 749-756
    CrossrefPubMedGoogle Scholar
  • 11 Ferreira T, Rasband W. ImageJ User Guide: IJ 1.45m (2011). Available at: http://rsbweb.nih.gov/ij/docs/user-guide.pdf . Accessed October 15, 2011
    PubMedGoogle Scholar
  • 12 Sclafani AP, Saman M. Platelet-rich fibrin matrix for facial plastic surgery. Facial Plast Surg Clin North Am 2012; 20 (2) 177-186 , vi
    CrossrefPubMedGoogle Scholar
  • 13 Choukroun J, Diss A, Simonpieri A , et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 101 (3) 299-303
    CrossrefPubMedGoogle Scholar
  • 14 Simon BI, Zatcoff AL, Kong JJW, O'Connell SM. Clinical and histological comparison of extraction socket healing following the use of autologous platelet-rich fibrin matrix (PRFM) to ridge preservation procedures employing demineralized freeze dried bone allograft material and membrane. Open Dent J. 2009; 3: 92-99
    CrossrefPubMedGoogle Scholar
  • 15 Dohan DM, Choukroun J, Diss A , et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 101 (3) e37-e44
    Google Scholar
  • 16 Monaco JL, Lawrence WT. Acute wound healing an overview. Clin Plast Surg 2003; 30 (1) 1-12
    CrossrefPubMedGoogle Scholar
  • 17 Tsirogianni AK, Moutsopoulus NM, Moutsopoulos HM. Wound healing: immunological aspects. Injury 2006; 37 (Suppl. 01) S5-S12
    PubMedGoogle Scholar
  • 18 Triana RJ, Murakami CS, Larrabee WF. Skin graft and local flap. In: Ira DP, Frodel J, Holt GR, Larrabee WF, Nathan N, Park SS, , et al, eds. Facial Plastic and Reconstructive Surgery. 3rd ed. New York, NY: Thieme; 2008: 41-58
    Google Scholar