Profiling of mushrooms using a UHPLC/UV/ELSD/HRMS dereplication protocol: A component of the safety assessment process

T El-Elimat; TN Graf; TR Baker; JG Little; NH Oberlies

doi:10.1055/s-0034-1382650

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Planta Med 2014; 80 - PPL14
DOI: 10.1055/s-0034-1382650

Profiling of mushrooms using a UHPLC/UV/ELSD/HRMS dereplication protocol: A component of the safety assessment process

T El-Elimat ¹, TN Graf ¹, TR Baker ², JG Little ³, NH Oberlies ¹

¹Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC 27402
²The Procter & Gamble Company, Mason, OH
³New Chapter, Inc., Brattleboro, VT

Congress Abstract

One challenge with safety assessment of fungi is confirmation that certain compounds, with known deleterious properties, produced by some species, are not present in samples. Harvesting fungal myceia under unique conditions, where potential production of mycotoxins is not well understood, amplifies this challenge. By utilizing high-resolution MS, MS/MS, and UV data and comparing to a database of over 200 fungal secondary metabolites, including mycotoxins, organic extracts of mushroom samples were dereplicated. None of the known mycotoxins were detected in the analyzed samples. Additionally, UHPLC using ELSD and HRMS chromatograms of the analyzed mushrooms were compared to each other and to two samples of edible mushrooms available in retail outlets. These data showed interesting similarities across all the samples. These data illustrate the utility of UHPLC/UV/ELSD/HRMS experiments for obtaining information that supports safety assessments on mushrooms consumed as nutritional supplements.