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Synlett 2015; 26(19): 2611-2616
DOI: 10.1055/s-0034-1378878
DOI: 10.1055/s-0034-1378878
synpacts
Synthesis of Metabolic Amino acid Precursors: Tools for Selective Isotope Labeling in Cell-Based Protein Overexpression
Weitere Informationen
Publikationsverlauf
Received: 29. Juni 2015
Accepted: 07. Juli 2015
Publikationsdatum:
12. August 2015 (online)
Abstract
The rapid development of biomolecular NMR spectroscopy in the last decade(s) has paved the way for novel insights into the structure, dynamic properties, as well as the interaction of proteins. However, NMR data interpretation of large protein complexes is only feasible if the corresponding sample is selectively enriched in NMR active nuclei (13C and 15N) and/or 1H-depleted by 2H incorporation. One important strategy for selective protein isotope labeling is given by the addition of metabolic amino acid precursor compounds to the growth medium of a protein-overexpressing microorganism. Herein, we highlight the synthetic concepts to access these precursors from commercially available isotope sources.
1 Introduction
2 Aliphatic Residue Precursors
3 Aromatic Residue Precursors
4 Precursors of Other Labeled Residues
5 Conclusions
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