Planta Med 2013; 79 - PJ20
DOI: 10.1055/s-0033-1352224

Phenolic Compounds from the Flower of Stewartia pseudo-camellia and Their Inhibitory Effects on the Release of β-Hexosaminidase in RBL-2H3 Cells

S Ha 1, Y Choi 1, Y Jeon 1, S Kang 2, O Zee 1, J Kwak 1
  • 1Sungkyunkwan University, School of Pharmacy, Suwon, Gyeonggi-do 440 – 746, South Korea.
  • 2Gachon University, College of Bionano, Seongnam, Gyeonggi-do 461 – 701, South Korea

Stewartia pseudo-camellia Maxim. (=S. koreana Nakai) is a deciduous tree in the family Theaceae which is distributed in southern Korea [1]. Its fruit or bark of stem and root have been used as a folk medicine for the treatment of circulatory disorders, paralysis of the limbs, legs and arms, and several pains. Previous studies on S. pseudo-camellia, have investigated several biological activities including angiogenesis [2], anti-inflammation [3, 4], prevention and treatment of osteoporosis [5], and anti-oxidative effect [6]. The flower of S. pseudo-camellia was consecutively extracted with hexane and MeOH. The anti-allergic activity of MeOH extract was comparatively higher than that of second-generation H1-antihistamine ketotifen as positive control. A new compound together with nine known compounds, (+)-catechin, quercetin-3-rhamnoside, methyl gallate, (-)-epicatechin, afzelin, aromadendrin, myricetin, kaempferol-7-O-rhamnosyl-3-O-rutinoside, and kaempferol-7-O-(4""-O-acetylrhamnosyl)-3-O-rutinoside were isolated from the MeOH extract. The structure of a new phenolic compound, named pseudocamellianin A, was determined as kaempferol-7-O-(4""-O-acetylrhamnosyl)-3-O-(4"'-O-p-coumaroyl)rutinoside from its spectral data. The anti-allergic activity of isolated compounds was evaluated in an in vitro assay on β-hexosaminidase release inhibition using RBL-2H3 cells stimulated by DNP-BSA. Among tested compounds, quercetin-3-rhamnoside, afzelin, aromadendrin, myricetin exhibited similar activity with ketotifen.

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References:

[1] Lee, TB. (1999) Illustrated flora of Korea. Hyangmoonsa. Seoul.

[2] Lee, TH et al. (2010) Phytother. Res. 24: 20 – 25.

[3] Lee, TH et al. (2012) International Immunopharmacology 13: 264 – 270.

[4] Jung, M-R et al. (2012) Biochemical and Biophysical Research Communications 427: 236 – 241.

[5] Park, CK et al. (2007) International Immunopharmacology 7: 1507 – 1516.

[6] Lee, SI et al. (2010) Biomolecules & Therapeutics 18: 191 – 196.