Secondary metabolites of Hypoxylon investiens, an endophytic fungus derived from Litsea akoensis var. chitouchiaoensis

IS Chen; CW Chang; MJ Cheng; GF Yuan; SY Hsieh

doi:10.1055/s-0033-1352109

Subscribe to RSS

Please copy the URL and add it into your RSS Feed Reader.

https://www.thieme-connect.de/rss/thieme/en/10.1055-s-00000058.xml

Share / Bookmark

Facebook X Linkedin Weibo

Planta Med 2013; 79 - PI19
DOI: 10.1055/s-0033-1352109

Secondary metabolites of Hypoxylon investiens, an endophytic fungus derived from Litsea akoensis var. chitouchiaoensis

IS Chen ¹, CW Chang ¹, MJ Cheng ², GF Yuan ², SY Hsieh ²

¹Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University (KMU), Kaohsiung 807, Taiwan.
²Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 300, Taiwan.

Congress Abstract

Many secondary metabolites, e.g. terpenoids, polyketides, cytochalasin, tetralone, were reported on the Hypoxylon sp. (Xylariaceae). In this study we focus on an endophytic fungus-Hypoxylon investiens BCRC 10F0115, isolated from the stem of an endemic plant, Litsea akoensis Hayata var. chitouchiaoensis Liao (Lauraceae). The aim of this study was the isolation of the n-BuOH extract of its metabolites and the evaluation of their bioactivities. So far, three new compounds, hypoxyloamide (1), 8-methoxynaphthalene1,7-diol (2), hypoxylonol (3) and five compounds first isolated from nature sources, investiamide (4), hypoxylopropanamide (5), (S)-5-methyl-8-O-methylmellein (6), hypoxylakolone (7), and hypoxylakolone A (8), together with 16 known compounds (9 – 24). Among these isolates, 8-methoxy-naphthalen-1-ol (17) and 1,8-dimethoxy-naphthalene (18) showed anti-inflammatory activities to inhibit iNOS, NO, and IL-6 production in LPS-activated RAW 264.7 cell, and 17 showed the stronger NO inhibitory activity at 10 µg/ml than the positive control quercetin, with IC₅₀value of 17.8µM without cytotoxicity.