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DOI: 10.1055/s-0033-1349852
Anti-obesity Effect of Gymnema sylvestre Extract on High Fat Diet-induced Obesity in Wistar Rats
Publikationsverlauf
received 12. April 2013
accepted 19. Juni 2013
Publikationsdatum:
10. Juli 2013 (online)
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Abstract
Gymnema sylvestre R. BR. (Asclepiadaceae) has been used frequently in traditional Indian folk medicine for the treatment of diabetes. Study was performed in high fat diet (HFD)-induced obesity in murine model. Obesity was induced by oral feeding of HFD for 28 days. The anti obesity effect of water soluble fraction of Gymnema sylvestre extract (120 mg/kg, p.o. for 21 days) in HFD fed rats was evaluated by the measurement of body weight gain, food intake, hemodynamic changes (systolic, diastolic, mean blood pressure and heart rate), serum lipid profiles (triglycerides, total cholesterol, LDL-cholesterol, HDL-cholesterol), leptin, insulin, glucose, apolipoproteins A1 and B, lactate dehydrogenase (LDH) and antioxidant enzymes such as reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-S transferase (GST), superoxide dismutase (SOD) and catalase (CAT) levels in liver tissues. Organs and visceral fat pad weight were measured. Histopathological studies were also carried out. Water soluble fraction of G. sylvestre ethanolic extract and rimonabant significantly reduced serum lipids, leptin, insulin, glucose, apolipoprotein B and LDH levels while it significantly increased the HDL-cholesterol, apolipoprotein A1 and antioxidant enzymes levels in liver tissue as compared to the HFD fed rats. Histopathological studies of tissues showed no pathological changes. The results of this study show that water soluble fraction of G. sylvestre extract possess antiobesity effect.
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References
- 1 Flier JS. Obesity wars: molecular progress confronts an expanding epidemic. Cell 2004; 116: 337-350
- 2 Flegal KM, Carroll MD, Ogden CL et al. Prevalence and trends in obesity among US adults, 1999–2000. JAMA 2002; 288: 1723-1727
- 3 American Heart Association . Heart Disease and Stroke Statistics – 2005 Update. Dallas TX: American Heart Association; 2005
- 4 Rippe JM, Crossley S, Ringer R. Obesity as a Chronic Disease: Modern Medical and lifestyle management. J Am Diet Asso 1998; 98: S9-S15
- 5 Dietz WM, Goodwin NJ, Yanovski SZ. Long-term pharmacotherapy in the management of obesity. JAMA 1996; 276: 1907-1915
- 6 Asayama K, Nakane T, Dobashi K et al. Effect of obesity and troglitazone on expression of two glutathione peroxidases: cellular and extracellular types in serum, kidney and adipose tissue. Free Rad Res 2001; 34: 337-347
- 7 Sies H. Strategies of antioxidant defense. Euro J Biochem 1993; 215: 213-219
- 8 Haller CA, Benowitz NL. Adverse cardiovascular and central nervous system events associated with dietary supplements containing ephedra alkaloids. New Eng J Med 2000; 343: 1833-1838
- 9 Nadkarni AK. Indian Material Medica. Vol. 1. Popular Prakashan Pvt, Ltd.; Bombay: 1982: 596-599
- 10 Bishayee A, Chatterjee M. Hypolipidemic and antiatherosclerotic effects of Gymnema sylvestre leaf extract in albino rats fed on high fat diet. Phytother Res 1994; 8: 118-120
- 11 Rachh PR, Rachh MR, Ghadiya NR et al. Antihyperlipidemic activity of Gymnema sylvestre R. Br. Leaf extract on rats fed with high cholesterol diet. Int J Pharmacol 2010; 6: 138-141
- 12 Shanmugasundaram KR, Paneerselvam C, Samudram P et al. Enzyme changes and glucose utilization in diabetic rabbits: the effect of Gymnema sylvestre. J Ethnopharmacol 1983; 7: 205-234
- 13 Shanmugasundaram ERB, Gopinath KL, Shanmugasundaram KR et al. Possible regeneration of the islets of Langerhans in streptozocin-diabetic rats given Gymnema sylvestre leaf extracts. J Ethnopharmacol 1990; 30: 265-279
- 14 Sharma SR, Dwivedi SK, Swarup D. Hypoglycaemic and hypolipidemic effects of Cinnamom tamala Nees leaves. Indian J Exp Biol 1996; 34: 372
- 15 Kanetkar P, Singhal R, Kamat M. Recent advances in Indian herbal drug research. Gymnema sylvestre: A Memoir. J Clin Biochem Nutr 2007; 41: 77-81
- 16 Alam MMA, Javed K, Jafri MA. Effect of Rheum emodi (Revand Hindi) on renal functions in rats. J Ethnopharmacol 2005; 96: 121-125
- 17 Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959; 82: 70-77
- 18 Mohandas J, Marshall JJ, Duggin GG et al. Low activities of glutathione-related enzymes as factors in the genesis of urinary bladder cancer. Cancer Research 1984; 44: 5086-5090
- 19 Calberg I, Mannerviek B. Glutathione reductase levels in rat brain. J Biol Chem 1975; 250: 5475-5480
- 20 Habig WH, Pabst MJ, Jakoby WB. Glutthione-S-transferase. The first enzymatic step in mercaptouric acid formation. J Biol Chem 1974; 249: 7130-7139
- 21 Marklund S, Marklund G. Involvement of the superoxide anion radical in the auto-oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974; 47: 469-474
- 22 Clairborne A. Catalase activity. In Greenwald RA. eds. Handbook of Methods for Oxygen Radical Research. Boca Raton: CRC Press; 1985: 283-284
- 23 Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351-358
- 24 Bonting SL. Sodium potassium activated adenosine triphosphatase and cation transport. In membrane and ion transport. Bitler EE. (ed.) Vol- 1. Interscience Wiley; London: 1970: 257-263
- 25 Folch J, Lees M, Stanley GHS et al. A simplified method for the isolation and purification of total lipids from animal tissues. J Biol Chem 1957; 226: 497-509
- 26 Kaur G, Kulkarni SK. Antiobesity effect of a polyherbal formulation, OB-200 G in female rats fed on cafeteria and atherogenic diets. Ind J Pharmacol 2000; 32: 294-299
- 27 Rexrode KM, Manson JE, Hennekens CH. Obesity and cardiovascular disease. Curr Opin Cardiol 1996; 11: 490-495
- 28 McNamara DJ. Dietary cholesterol and atherosclerosis. Biochem Biophysics Acta 2000; 1529: 310-320
- 29 Kaufman LN, Peterson MM, Smith SM. Hypertension and sympathetic hyperactivity induced in rats by high-fat or glucose diets. Am J Physiol 1991; 260: E95-E100
- 30 Lavie CJ, Milani RV. Obesity and cardiovascular disease: the Hippocrates paradox?. J Am Coll Cardiol 2003; 42: 677-679
- 31 Jayakumar SM, Nalini N, Venugopal PM. Effect of ginger (Zingifer officinale) on lipids in rats fed atherogenic diet. J Clin Biochem Nutr 1991; 27: 79-82
- 32 Hyson DA, Schneeman BO, Davis PA. Almonds and almond oil have similar effects on plasma lipids and LDL oxidation in healthy men and women. J Nutr 2002; 132: 703-707
- 33 Fried SK, Ricci MR, Russell CD et al. Regulation of leptin production in humans. J Nutr 2000; 130: 3127S-3131S
- 34 Uygun A, Kadayifci A, Yesilova Z et al. Serum leptin levels in patients with nonalcoholic steatohepatitis. Am J Gastroenterol 2000; 95: 3584-3589
- 35 D’Souza T, Mengi SA, Hassarajani S et al. Efficacy study of the bioactive fraction (F-3) of Acorus calamus in hyperlipidemia. Indian J Pharmacol 2007; 39: 196-200
- 36 Festi D, Colecchia A, Sacco T et al. Hepatic steatosis in obese patients: clinical aspects and prognostic significance. Obesity Reviews 2004; 5: 27-42
- 37 Olusi SO. Obesity is an independent risk factor for plasma lipid peroxidation and depletion of erythrocyte cytoprotectic enzymes in humans. Int J Obes Relat Metab Dis 2002; 26: 1159-1164
- 38 Ozata M, Mergen M, Oktenli C et al. Increased oxidative stress and hypozincemia in male obesity. Clin Biochem 2002; 35: 627-631
- 39 Prakash J, Gupta SK, Kochupillai V et al. Chemopreventive activity of Withania somnifera in experimentally induced fibrosarcoma tumours in Swiss albino mice. Phytother Res 2001; 15: 240-244
- 40 Husain K, Mejia J, Lalla J et al. Dose response of alcohol-induced changes in BP, nitric oxide and antioxidants in rat plasma. Pharmacol Res 2005; 51: 337-343
- 41 Nicolle C, Cardinault N, Aprikian O et al. Effect of carrot intake on cholesterol metabolism and on antioxidant status in cholesterol-fed rat. Euro J Nutr 2003; 42: 254-261
- 42 Ko JH, Lee SJ, Lim KT. 36-kDa glycoprotein isolated from Rhus verniciflua Stokes fruit has a protective activity to glucose/glucose oxidase-induced apoptosis in NIH/3T3 cells. Toxicology in Vitro 2005; 19: 353-363
- 43 Liou W, Chang LY, Geuze HJ et al. Distribution of CuZn superoxide dismutase in rat liver. Free Radical Biology and Medicine 1993; 14: 201-207
- 44 Mehta K, Van Thiel DH, Shah N et al. Nonalcoholic fatty liver disease: pathogenesis and the role of antioxidants. Nutrition Reviews 2002; 60: 289-293