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Horm Metab Res 2013; 45(12): 887-892
DOI: 10.1055/s-0033-1347200
Original Basic
© Georg Thieme Verlag KG Stuttgart · New York

17β-Estradiol Reduces Nitric Oxide Production in the Guinea Pig Cochlea

U.-R. Heinrich
1   Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
,
J. Brieger
1   Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
,
C. Striedter
1   Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
,
I. Fischer
1   Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
,
I. Schmidtmann
2   Institute for Medical Statistics, Epidemiology and Informatics (IMBEI), University Medical Center of the Johannes ­Gutenberg University Mainz, Mainz, Germany
,
H. Li
3   Department of Pharmacology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
,
W. J. Mann
1   Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
,
K. Helling
1   Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
› Author Affiliations
Further Information

Publication History

received 25 April 2013

accepted 06 May 2013

Publication Date:
21 June 2013 (online)

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Abstract

Intense noise exposure and the application of ototoxic substances result in increased levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS), such as nitric oxide (NO). In order to reduce the free NO concentration in the inner ear under pathological conditions, the use of natural cytoprotective substances such as 17β-estradiol is a promising therapeutic concept. In male guinea pigs the organ of Corti and the lateral wall were isolated from the cochlea and afterwards incubated for 6 h in cell-culture medium. 17β-Estradiol was adjusted in 2 concentrations to organ cultures of the right ears (12 animals per concentration). The left ears were used as controls. The NO production was quantified in the supernatant by chemiluminescence after incubation. Depending on the concentration, 17β-estradiol reduced NO in the organ of Corti by 43% (p=0.015) and 46% (p=0.026), respectively. In the lateral wall, the NO concentration was reduced by 24%, but without statistical significance (p=0.86). However, when analyzing the association between the 2 cochlear regions for each animal separately, the NO concentrations were lower in nearly all 17β-estradiol-treated ears compared to controls. In order to demonstrate the flexibility of the organ culture system, the NO donor DETA NONOate and the nitric oxide synthase inhibitors L-NAME and L-NMMA were applied. The electron microscopic analysis revealed a well-preserved cochlear cell morphology after incubation. The ability of 17β-estradiol to influence the NO production preferentially in the organ of Corti might offer new therapeutic perspectives for inner ear protection.

Key words

inner ear - organ culture - cytoprotection - lateral wall - organ of Corti
 

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