RSS-Feed abonnieren
DOI: 10.1055/s-0033-1343644
The transcription factor activating protein 2 beta (TFAP2B) mediates neuronal differentiation in neuroblastoma cells
We here aimed to investigate the role of the transcription factor activating protein 2 beta (TFAP2B) in neuroblastoma pathogenesis. TFAP2 is a family of transcription factors that play a crucial role in embryonic differentiation and development. Oligonucleotide-microarray analysis of 649 neuroblastoma specimens demonstrated that TFAP2B downregulation is associated with unfavourable prognostic markers such as disseminated stage 4, age > 18 months, MYCN amplification, unfavourable gene expression-based classification and adverse patient outcome.
Tetracycline inducible re-expression of TFAP2B in neuroblastoma cells significantly impaired proliferation and led to arrest in the G1-phase of the cell cycle in IMR-32 and SH-EP cells. Neuronal differentiation and senescence was observed in IMR-32 and SH-EP cells, respectively, after TFAP2B induction. In addition, knockdown of TFAP2B by lentiviral transduction of shRNA abrogated retinoic acid induced neuronal differentiation of SH-SY5Y and SK-N-Be(2)-C cells. Taken together, our results point towards a role of TFAP2B in neuronal differentiation of neuroblastoma.