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Drug Res (Stuttg) 2013; 63(02): 65-68
DOI: 10.1055/s-0032-1331754
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Broad Spectrum Antibacterial Activity of a Mixture of Isothiocyanates from Nasturtium (Tropaeoli majoris herba) and Horseradish (Armoraciae rusticanae radix)

A. Conrad
1   Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany
,
D. Biehler
1   Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany
,
T. Nobis
1   Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany
,
H. Richter
1   Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany
,
I. Engels
1   Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany
,
K. Biehler
1   Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany
,
U. Frank
1   Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany
› Author Affiliations
Further Information

Publication History

received 19 April 2012

accepted 26 November 2012

Publication Date:
17 January 2013 (online)

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Abstract

Isothiocyanates have been reported to exert antimicrobial activity. These compounds are found in a licensed native preparation of nasturtium (Tropaeoli majoris herba) and horseradish (Armoraciae rusticanae radix) which is used for treatment of upper respiratory and urinary tract infections. The aim of our investigation was to assess the antimicrobial activity of a mixture of the contained benzyl-, allyl-, and phenylethyl- isothiocyanates against clinically important bacterial and fungal pathogens including antimicrobial resistant isolates. Susceptibility testing was performed by agar-dilution technique. Isothiocyanates were mixed in proportions identical to the licensed drug. Minimum inhibitory- and minimum bactericidal concentrations were assessed. The Minimum inhibitory concentration90 was defined as the concentration which inhibited 90% of the microbial species tested. H. influenzae, M. catarrhalis, S. marcescens, P. vulgaris, and Candida spp. were found to be highly susceptible, with minimum inhibitory concentration90 -values ranging between ≤0.0005% and 0.004% (v/v) of total ITC. Intermediate susceptibilities were observed for S. aureus, S. pyogenes, S. pneumoniae, K. pneumoniae, E. coli and P. aeruginosa, with Minimum inhibitory concentration90 -values ranging between 0.004% and 0.125% (v/v), but with elevated Minimum bactericidal concentrations90-values (2–7 dilution steps above Minimum inhibitory concentration90). Low susceptibilities were determined for viridans streptococci and enterococci. Interestingly, both resistant and non-resistant bacteria were similarly susceptible to the test preparation.

Key words

isothiocyanate - Tropaeoli majoris herba - Armoraciae rusticanae radix - antimicrobial activity
 

  • References

  • 1 Ratzka A, Vogel H, Kliebenstein DJ et al. Disarming the mustard oil bomb. PNAS 2001; 99: 11223-11228
    PubMedSearch in Google Scholar
  • 2 Lamy E, Mersch-Sundermann V. Sulphur-containing compounds in food plants: kinetics, biological activity, and mechanisms of action. Environ Mol Mutagen 2009; 50: 163
    CrossrefPubMedSearch in Google Scholar
  • 3 Gaase A. Bakteriologische Empfindlichkeitstestungen eines Antibiotikums. Ärztl Prax 1968; 20: 665-668
    PubMedSearch in Google Scholar
  • 4 Germer W. Ein neues Präparat für die antibiotische Therapie auf pflanzlicher Grundlage. Dtsch Med Wochenschr 1954; 79: 1445-1458
    Thieme ConnectPubMedSearch in Google Scholar
  • 5 Halbeisen T. Untersuchungen des antibiotischen Wirkstoffs einer höheren Pflanze (Tropaeolum maius – Kapuzinerkresse). Medizinische 1954; 36: 1212-1215
    PubMedSearch in Google Scholar
  • 6 Halbeisen T. Untersuchungen über die antibiotischen Wirkstoffe von Tropaeolum majus (Kapuzinerkresse). Naturwissenschaften 1954; 41: 378-379
    PubMedSearch in Google Scholar
  • 7 Halbeisen T. Eine antibiotische wirksame Substanz aus Cochlearia Armoracia L . Arzneimittelforscung 1957; 7: 321-323
    PubMedSearch in Google Scholar
  • 8 Kienholz M, Kemkes B. Die antibakterielle Wirkung ätherischer Öle aus Meerrettich-Wurzeln. Arzneimittelforschung 1960; 10: 917-918
    PubMedSearch in Google Scholar
  • 9 Klesse P, Lukoschek P. Untersuchungen über die bakteriostatische Wirksamkeit einiger Senföle. Arzneimittelforschung 1955; 5: 505-507
    PubMedSearch in Google Scholar
  • 10 Orzechowski G. Antimikrobiotika in höheren Pflanzen und die Problematik der Therapie mit Antibiotika. Pharm Rundsch 1961; 3: 41-47
    PubMedSearch in Google Scholar
  • 11 Rudat KD, Loepelmann JM. Über die bakterienhemmende Wirkung der in der Kapuzinerkresse (Tropaeolum maius) enthaltenen antibiotischen Stoffe, insbesondere gegenüber aeroben Sporenbildnern. Pharmazie 1955; 10: 729-732
    PubMedSearch in Google Scholar
  • 12 Schaffer RJ. Untersuchungen zur in vitro-Wirksamkeit von Benzylsenföl gegen pathogene Bakterien und Sprosspilze [dissertation]. 1980 University of Cologne, Germany
    PubMedSearch in Google Scholar
  • 13 Stickl H. Über eine antibiotisch wirksame Pflanzensubstanz. Dtsch Med Wochenschr 1954; 79: 1722-1725
    Thieme ConnectPubMedSearch in Google Scholar
  • 14 Winter AG, Willeke L. Untersuchungen über Antibiotica aus höheren Pflanzen: VI. Mitteilung: Gasförmige Hemmstoffe aus Tropaeolum maius und ihr Verhalten im menschlichen Körper bei Aufnahme von Tropaeolum-Salat per os. Naturwissenschaften 1952; 39: 236-237
    CrossrefPubMedSearch in Google Scholar
  • 15 Winter AG, Hornbostel M. Untersuchungen über Antibiotika aus höheren Pflanzen: IX. Mitteilung: Gasförmige Hemmstoffe aus Cochlearia armoracia (Meerrettich) und ihr Verhalten im menschlichen Körper bei Aufnahme per os. Naturwiss 1953; 40: 489-490
    PubMedSearch in Google Scholar
  • 16 Winter AG. Antibiotische Therapie mit Arzneipflanzen. Planta Med 1955; 3: 1-16
    Thieme ConnectPubMedSearch in Google Scholar
  • 17 Winter AG. Die Bedeutung ätherischer Öle für die Behandlung von Harnwegsinfektionen. Planta Med 1958; 6: 306-313
    PubMedSearch in Google Scholar
  • 18 Aires A, Mota VR, Saavedra MJ et al. The antimicrobial effects of glucosinolates and their respective enzymatic hydrolysis products on bacteria isolated from the human intestinal tract. J Appl Microbiol 2009; 106: 2086-2095
    CrossrefPubMedSearch in Google Scholar
  • 19 Isshiki K, Tokuoka K, Mori R et al. Preliminary examination of allyl isothiocyanate vapor for food preservation. Bioscience, Biotechnology and Biochemistry 1992; 9: 1476-1477
    PubMedSearch in Google Scholar
  • 20 Lin CM, Kim JM, Du WX et al. Bactericidal activity of isothiocyanate against pathogens on fresh produce. Journal of Food Protection 1999; 62: 5100-5107
    PubMedSearch in Google Scholar
  • 21 Luciano FB, Holley RA. Enzymatic inhibition by allyl isothiocyanate and factors affecting its antimicrobial action against Escherichia coli O157:H7. Int J Food Microbiol 2009; 131: 240-245
    CrossrefPubMedSearch in Google Scholar
  • 22 Rhee MS, Lee SY, Dougherty RH et al. Antimicrobial effects of mustard flour and acetic acid against Escherichiacoli O157:H7, Listeria monocytogenes and Salmonella enterica serovar Typhimurium . Applied and Environmental Microbiology 2003; 69: 2959-2963
    CrossrefPubMedSearch in Google Scholar
  • 23 Goos KH, Albrecht U, Schneider B. Efficacy and safety profile of a herbal drug containing nasturtium herb and horseradish root in acute sinusitis, acute bronchitis and acute urinary tract infection in comparison with other treatments in the daily practice/results of a prospective cohort study. Arzneimittelforschung 2006; 56: 249-257
    Thieme ConnectPubMedSearch in Google Scholar
  • 24 Goos KH, Albrecht U, Schneider B. On-going investigations on efficacy and safety profile of a herbal drug containing nasturtium herb and horseradish root in acute sinusitis, acute bronchitis and acute urinary tract infection in children in comparison with other antibiotic treatments. Arzneimittelforschung 2007; 57: 238-246
    Thieme ConnectPubMedSearch in Google Scholar
  • 25 Albrecht U, Goos KH, Schneider B. A randomised, double-blind, placebo-controlled trial of a herbal medicinal product containing Tropaeoli majoris herba (Nasturtium) and Armoraciae rusticanae radix (Horseradish) for the prophylactic treatment of patients with chronically recurrent lower urinary tract infections. Curr Med Res Opin 2007; 23: 2415-2422
    CrossrefPubMedSearch in Google Scholar
  • 26 Harkenthal M, Reichling J, Geiss HK et al. Comparative study on the in vitro antibacterial activity of Australian tea tree oil, cajuput oil, niaouli oil, manuka oil, kanuka oil, and eucalyptus oil. Pharmazie 1999; 54: 460-463
    PubMedSearch in Google Scholar
  • 27 Meyer E, Schwab F, Jonas D et al. Surveillance of antimicrobial use and antimicrobial resistance in intensive care units (SARI): 1. Antimicrobial use in German intensive care units. Intensive Care Med 2004; 30: 1089-1096
    CrossrefPubMedSearch in Google Scholar
  • 28 Conrad A, Kolberg T, Engels I et al. In vitro study to evaluate the antibacterial activity of a combination of the haulm of nasturtium (Tropaeoli majoris herba) and of the roots of horseradish (Armoraciae rusticanae radix). Arzneimittelforschung 2006; 56: 842-849
    Thieme ConnectPubMedSearch in Google Scholar
  • 29 Kawakishi S, Kaneko T. Interaction of oxidized glutathione with allyl isothiocyanate. Phytochemistry 1985; 24: 715-718
    CrossrefPubMedSearch in Google Scholar
  • 30 Kawakishi S, Kaneko T. Interaction of proteins with allyl isothiocyanate. Journal of Agriculture and Food Chemistry 1987; 35: 85-88
    CrossrefPubMedSearch in Google Scholar
  • 31 Livermore DM. Has the era of untreatable infections arrived?. J Antimicrob Chemother 2009; 64 (Suppl. 01) 29-36
    CrossrefPubMedSearch in Google Scholar
  • 32 Kawakishi S, Namiki M. Decomposition of allyl isothiocyanate in aqueous solution. Agricultural and Biological Chemistry 1969; 33: 452-459
    CrossrefPubMedSearch in Google Scholar