Planta Med 2012; 78 - PJ60
DOI: 10.1055/s-0032-1321220

Metabolomic approach for origin discrimination of Anemarrhena asphodeloides bunge using UPLC/Q-TOF MS

S Ryu 1, N Kim 1, W Jeong 1, D Lee 2, JW Nam 3, UJ Youn 3, JW Lee 2, EK Seo 3, JH Lee 4, D Lee 1
  • 1School of Life Sciences and Biotechnology, Korea University, Seoul 136–713, Korea
  • 2Department of Statistics, Korea University, Seoul 136–701
  • 3College of Pharmacy and Center for Cell Signaling & Drug Discovery Research, Ewha Womans University, Seoul 120–750
  • 4College of Oriental Medicine, Dongguk University, Gyeongju 780–714

An ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-Tof MS) analytical method has been developed for metabolite profiling and pattern analysis of Anemarrhena asphodeloides Bunge. Total 21 extracts of A.asphodeloides bunge from different origins were analyzed by UPLC/Q-Tof MS. In addition, quality control samples were also analyzed to validate the analytical method and to ensure more reliable and accurate results. Subsequently, multivariate statistical analyses were performed to compare the patterns among tested samples. Furthermore, statistic methods, t-test and significance analysis of microarrays, were applied to extract influential metabolites for the efficient comparison of two groups. As a result, PCA and HCA with the selected metabolites clearly showed origin discrimination of A.asphodeloides bunge.