Planta Med 2012; 78 - PI331
DOI: 10.1055/s-0032-1321018

Bioassay-guided isolation of anti-Leishmania major compounds from Micromelum minutum

A Sakunpak 1, K Matsunami 2, H Otsuka 2, P Panichayupakaranant 1, 3
  • 1Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand
  • 2Department of Pharmacognosy, Graduate School of Biomedical Sciences, Hiroshima University; 1–2-3 Kasumi, Minamiku, Hiroshima 734–8553, Japan
  • 3Phytomedicine and Pharmaceutical Biotechnology Research Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand

Anti-Leishmania major assay-guided isolation of Micromelum minutum leaves gave two new monoterpene coumarins, named minutin A and minutin B, together with four known coumarins, 8,4′′-dihydroxy-3′′,4′′-dihydrocapnolactone-2′,3′diol, 8-hydroxyisocapnolactone-2′,3′- diol, 8-hydroxy-3′′,4′′-dihydrocapnolactone-2′,3′-diol, and clauslactone E. Minutin A, minutin B, 8-hydroxyisocapnolactone-2′,3′-diol and clauslactone E showed satisfactory anti-L. major activity with IC50 of 26.2, 20.2, 12.1, and 9.8µM, respectively, while 8,4′′-dihydroxy-3′′,4′′-dihydrocapnolactone-2′,3′-diol and 8-hydroxy-3′′,4′′-dihydrocapnolactone-2′,3′-diol were not active.