Isolation characterization and anti-inflammatory activity of Rhaphiostylis beninensis Planch. Ex Benth (Icacinaceae)

J Ofeimun; B Ayinde; I Igbe; MI Choudhary; I Husain; A Adhikari

doi:10.1055/s-0032-1321014

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Planta Med 2012; 78 - PI327
DOI: 10.1055/s-0032-1321014

Isolation characterization and anti-inflammatory activity of Rhaphiostylis beninensis Planch. Ex Benth (Icacinaceae)

J Ofeimun ¹, B Ayinde ¹, I Igbe ², MI Choudhary ³, I Husain ³, A Adhikari ³

¹Department of Pharmacognosy, Faculty of Pharmacy, University of Benin, Benin City 300001, Nigeria
²Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
³H. E. J Research Institute, University of Karachi, Pakistan

Congress Abstract

Rhaphiostylis beninensis is used in traditional medicine in Nigeria as an anti-inflammatory crude drug. This claim was investigated through bioactivity- guided fractionation chromatography using the Carageenan induced Rat Paw inflammation model. A sulphonyl derivative: N,N -Bis [ (4-methoxyphenyl) methyl]thiourea was isolated and it's structure was elucidated using Mass Spectrometr and Nuclear Magnetic Resonance (HNMR and ¹³CNMR). The anti-inflammatory activities were enhanced with fractionation and subsequent chromatographic exercises. The occurance and anti-inflammatory activity of the compound are reported for the first time in Rhaphiostylis beninensis. The results confirm the traditional use of the plant.