In vitro anti-inflammatory and anti-proliferatIve effects of Prangos platychloena Boiss.ex Tchih. on colorectal cancer cell line

We investigated anti-carcinogenic and anti-inflammatory activities of Prangos platychloena plant. We monitored such activities in two different extracts from the plant, methanolic and water, at 10–1000µg/ml concentrations, in colorectal cancer cell line (CCL-221) and colon cancer cell line (Caco-2) using Trypan Blue Exclusion Test. We investigated the anti-inflammatory activity of the extracts on interleukin 8 (IL-8) and interleukin 6 (IL-6) secretion after stimulating the cancer cell by tumor necrosis factor-α (TNF-α). We used different concentrations of the plant, ranging from 10 to 1000µg/mL. We observed that the most effective anticancer activity was at 1000µg/ml concentration. At 1000µg/mL concentration, the water extract showed anti-carcinogenic properties with maximum inhibition of 72% in CCL-221 cell, and maximum inhibition of 59% in Caco-2 cell. We observed no cytotoxic effect against normal cell line (Human fibroblast).

Finally, we observed that IL-8 decreased from 519.07 pg/ml to 28.3 pg/ml with water extract of P.platychloena, to 92.73 pg/ml with its methanol extract in ccl_221, IL-6 decreased from 63 pg/ml to 1pg/ml with the water extract, and to 4 pg/ml with methanol extract. These results showed that P. platychloena could be used in pharmaceutical applications because of its remarkable anticancer and anti-inflammatory effects on CCL-221 and Caco-2 cancer cell lines.