New cromones from Cnidium monnieri fruits inhibit adipocyte differentiation in 3T3-L1 cells

Seven new chromone glycosides, monnieriside A (3), B (10), C (12) D (13), E (15), F (16), G (17) and one new phenolic compound, methylpicraquassioside B (23) were isolated from the n-BuOH soluble fraction of Cnidium monnieri fruits (Umbelliferae), together with fourteen known compounds, undulatoside A (1), cnidimol C (2), saikochromoside A (4), cnidimoside A (5), cnidimoside B (6), 2-methyl-5-hydroxy-6-(2-butenyl-3-hydroxymethyl)-7-(β-D-glucopyranosyloxy)-4H-1-benzopyran-4-one (7), cnidimol D (8), hydroxycnidimoside A (9), umtatin (11), 6′-hydroxylangelicain (14), cnidioside A (18), cnidioside B (19), picraquassioside A (20), methylpicraquassioside A (21) and picraquassioside B (22). The structures of isolated compounds were determined on the basis of spectroscopic analysis including 1D, 2D NMR and HRESI-MS. Among the compounds isolated, compounds 5, 6, 9 and 10 significantly inhibited adipocyte differentiation as measured by fat accumulation in 3T3-L1 cells using Oil Red O staining.