Phytochemical screening, isolation of betulinic acid and trigonelline from stems of an indigenous plant, from the Guyana flora

RC Jagessar; G Hoolas; AR Maxwell

doi:10.1055/s-0032-1320858

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Planta Med 2012; 78 - PI170
DOI: 10.1055/s-0032-1320858

Phytochemical screening, isolation of betulinic acid and trigonelline from stems of an indigenous plant, from the Guyana flora

RC Jagessar ¹, G Hoolas ¹, AR Maxwell ²

¹Department of Chemistry, University of Guyana, South America
²Department of Chemistry, University of the West Indies, St. Augustine campus, Trinidad and Tobago

Congress Abstract

The stems of an indigenous plant of the Guyana (South America) flora were screened for natural products using solvents of varying polarity: C₆H₁₂, CH₂Cl₂, EtOAc, CH₃CH₂OH. Phytochemical screening revealed the presence of Emodols, Tannins, Flavones and other reducing compounds in the non-hydrolysed C₂H₅OH extract. C₆H₁₂ extracts revealed the presence of sterols, triterpenes, coumarins and reducing compounds. From the CH₂Cl₂ extract, a white solid crystallized, which after further purification and spectroscopic elucidation, yielded the lupine type pentacyclic triterpene, betulinic acid (1) (betulinic acid (3-beta-hydroxylup-20-(29)-en-28-oic acid). The EtOAc extract showed the presence of emodols, tannins, flavones, reducing compounds and alkaloid salt. Flash column chromatography yielded Trigonelline (2) as one of the major fraction. For the hydrolysed C₂H₅OH extract, positive tests were noted for anthrasenosides and coumarins. The non-hydrolysed C₂H₅OH extract revealed the presence of emodols, tannins, reducing compounds and flavones.