Subscribe to RSS
DOI: 10.1055/s-0032-1320837
Analysis of chromone glycosides in saposhnikovia divaricata for the establishment of a monograph of the german pharmacopoeia
Medicinal plants from Traditional Chinese Medicine (TCM) are getting popular in Europe. Therefore it was decided to develop TCM monographs for the German Pharmacopoeia (DAB). Saposhnikoviae divaricatae radix is used in TCM to treat common cold and headache. Prim-O-glucosylcimifugin and 4'-O-β-D-glucosyl-5-O-methylvisamminol are considered to be among the active compounds of Saposhnikovia divaricata (Turcz.) Schischk. Thus, these chromones were selected as suitable reference compounds for TLC/HPTLC fingerprint identification and quantitative analysis in the assay. A new solvent system allows the HPTLC differentiation of the medicinal herbal drug from its most common adulterants. For quantification, a HPLC method published by Xin was modified and validated, resulting in good separation of the chromones. In comparison, also a densitometric HPTLC method was developed for these compounds. Quantitative HPLC and HPTLC were compared to evaluate their suitability for a new Saposhnikovia monograph.