Novel Kaempferol triglycosides from Olax manni leaves: Structures and biological activities

Four (4) new kaempferol triglycosides, namely, Kaempferol 3-O- α-D- apiofuranosyl (1–- 2) α-L- arabinofuranosyl- 7-O- α-L- rhamnopyranoside (1), Kaempferol 3-O- β-D- glucopyranosyl (1–- 2) α- L- arabinofuranosyl- 7-O- α-L- rhamnopyranoside (2), Kaempferol 3-O- α-L- arabinofuranosyl (1–- 2)- β-D- galactopyranosyl- 7-O- α-L- rhamnopyranoside (3), Kaempferol 3-O- α-L- rhamnopyranosyl (1....2)- α-L- arabinofuranosyl- 7-O- α-L- rhamnopyranoside (4) in addition with fifteen (15) known flavonoid glycosides (5–19) were isolated from the leaves of Olax manni. All the compounds were isolated for the first time from the genus, Olax. The structures of these compounds were elucidated by HPLC, LC-ESIMS, ¹HNMR, ¹³CNMR, DEPT, HMQC and HMBC. The compounds showed moderate antifungal activity against Aspagillus fumigatus and mild cytotoxicity against mouse lymphoma cell line (L5178Y).