De Novo sequencing and characterization of developing seed transcriptome in two buckwheat species and metabolome profiling

Common buckwheat (Fagopyrum esculentum) is an important food crop while Tartary buckwheat is prominent for high amount of secondary metabolites. Metabolomics allows the simultaneous detection of a wide range of compounds, providing an immediate information of the metabolome of a plant. The most universally used metabolomic approaches comprises NMR and MS. They have been applied as a proof of principle to show that metabolomics can constitute a major advancement in the study of plant. So, we use NMR and MS instruments to discuss the changes in relations between the buckwheat leaf, seed and flower of the secondary metabolite. Here we also present the results of transcriptome sequencing of developing seeds for two closely related plant species, with special emphasis on their secondary metabolites. Using ABI SOLiD 5500 sequencing technology, approximately 30 millions of reads with average length of 75 nucleotides were sequenced. De novo assembly of the reads produced about 20 thousands of contigs for each species with 100X coverage. Comparative analysis of two transcriptomes and metabolomes will be presented.