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Synlett 2012; 23(12): 1840-1842
DOI: 10.1055/s-0032-1316554
DOI: 10.1055/s-0032-1316554
letter
Monitoring Biocatalytic Transformations Mediated by Polyketide Synthase Enzymes in Cell Lysate via Fluorine NMR
Further Information
Publication History
Received: 12 April 2012
Accepted after revision: 25 May 2012
Publication Date:
04 July 2012 (online)
![](https://www.thieme-connect.de/media/synlett/201212/lookinside/thumbnails/10.1055-s-0032-1316554-1.jpg)
Abstract
The biocatalytic employment of modular polyketide synthase enzymes in cell lysate has become a viable route to preparative quantities of synthetically valuable polyketide fragments. We report the quantitative, uninvasive, and continuous monitoring of such biocatalytic reactions by observing trifluoromethyl-bearing substrates via 19F NMR spectroscopic analysis. To demonstrate the utility of this technique, we followed reactions catalyzed by a thioesterase and several ketoreductases.
Supporting Information
- for this article is available online at http://www.thieme-connect.com/ejournals/toc/synlett.
- Supporting Information
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