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Am J Perinatol 2012; 29(06): 455-458
DOI: 10.1055/s-0032-1304827
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Comparison of Bactericidal Properties of Alcohol-Based Chlorhexidine versus Povidone-Iodine Prior to Amniocentesis

Michael T. Adler
1   Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Texas Medical School Houston, Houston, Texas
,
Kristin R. Brigger
1   Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Texas Medical School Houston, Houston, Texas
,
Karen D. Bishop
1   Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Texas Medical School Houston, Houston, Texas
,
Joan M. Mastrobattista
1   Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Texas Medical School Houston, Houston, Texas
› Author Affiliations
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Publication History

15 September 2011

24 November 2011

Publication Date:
07 March 2012 (online)

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Abstract

Objective To compare the bactericidal properties of povidone-iodine versus alcohol-based chlorhexidine solution for cleansing the gravid abdomen prior to amniocentesis.

Methods Fifty study participants were recruited from the University of Texas Women's Clinic in Houston, Texas. Two baseline swabs of the patients' abdomens were obtained to assess bacterial flora prior to treatment. A 10% povidone-iodine solution and 2% chlorhexidine gluconate with 70% isopropyl alcohol solution in a 3-mL prefilled applicator (Chloraprep, Cardinal Health, Inc., Leawood, KS) were then applied on different sides of the abdomen. After 30 seconds, cultures were obtained and plated on Trypticase Soy (PML Microbiologicals, Durham, NC) with sheep's blood agar for aerobic flora. Plates were incubated at 37°C for 48 hours for aerobic flora. Colony-forming units were counted and recorded.

Results No statistically significant difference was detected between baseline colony counts between the left and right side of each patient's abdomen (p = 0.33) prior to cleansing. Postcleansing colony counts were evaluated, and a statistically significant difference was identified, favoring chlorhexidine as a more efficacious abdominal cleanser (p <0.001).

Conclusion We demonstrated that 2% chlorhexidine with 70% isopropyl alcohol had excellent bactericidal efficacy and was superior to povidone-iodine for cleansing the maternal abdomen.

Keywords

abdominal - amniocentesis - intra-amniotic infection
 

  • References

  • 1 Invasive Prenatal Testing for Aneuploidy ACOG Practice Bulletin, Number 88, December 2007.
    PubMedGoogle Scholar
  • 2 National Cancer Institute website NCI drug dictionary. Available at: http://www.cancer.gov/DRUGDICTIONARY . Accessed May 11, 2009
    PubMedGoogle Scholar
  • 3 Mimoz O, Karim A, Mercat A , et al. Chlorhexidine compared with povidone-iodine as skin preparation before blood culture. A randomized, controlled trial. Ann Intern Med 1999; 131: 834-837
    PubMedGoogle Scholar
  • 4 Darouiche RO, Wall Jr MJ, Itani KM , et al. Chlorhexidine-alcohol versus povidone-iodine for surgical-site antisepsis. N Engl J Med 2010; 362: 18-26
    CrossrefPubMedGoogle Scholar
  • 5 Mulberrry G, Snyder AT, Heilman J, Pyrek J, Stahl J. Evaluation of a waterless, scrubless chlorhexidine gluconate/ethanol surgical scrub for antimicrobial efficacy. Am J Infect Control 2001; 29: 377-382
    CrossrefPubMedGoogle Scholar
  • 6 Hibbard JS, Mulberry GK, Brady AR. A clinical study comparing the skin antisepsis and safety of ChloraPrep, 70% isopropyl alcohol, and 2% aqueous chlorhexidine. J Infus Nurs 2002; 25: 244-249
    CrossrefPubMedGoogle Scholar
  • 7 Leung MP, Bishop KD, Monga M. The effect of temperature on bactericidal properties of 10% povidone-iodine solution. Am J Obstet Gynecol 2002; 186: 869-871
    CrossrefPubMedGoogle Scholar