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Horm Metab Res 2012; 44(01): 28-32
DOI: 10.1055/s-0031-1297942
Original Basic
© Georg Thieme Verlag KG Stuttgart · New York

Glycerol Metabolism Alteration in Adipocytes from n3-PUFA-Depleted Rats, an Animal Model for Metabolic Syndrome

L. Portois
1   Laboratory of Experimental Surgery L. Deloyers, Université Libre de Bruxelles, Brussels, Belgium
,
Y. Zhang
2   Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium
,
J. Perret
3   Laboratory of Biological Chemistry and Nutrition, Université Libre de Bruxelles, Brussels, Belgium
,
K. Louchami
2   Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium
,
N. Gaspard
3   Laboratory of Biological Chemistry and Nutrition, Université Libre de Bruxelles, Brussels, Belgium
,
E. Hupkens
2   Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium
,
N. Bolaky
3   Laboratory of Biological Chemistry and Nutrition, Université Libre de Bruxelles, Brussels, Belgium
,
V. Delforge
1   Laboratory of Experimental Surgery L. Deloyers, Université Libre de Bruxelles, Brussels, Belgium
,
R. Beauwens
4   Laboratory of Cell and Molecular Physiology, Université Libre de Bruxelles, Brussels, Belgium
,
W. J. Malaisse
2   Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium
,
A. Sener
2   Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium
,
Y. A. Carpentier
1   Laboratory of Experimental Surgery L. Deloyers, Université Libre de Bruxelles, Brussels, Belgium
,
C. Delporte
3   Laboratory of Biological Chemistry and Nutrition, Université Libre de Bruxelles, Brussels, Belgium
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 13. September 2011

accepted 14. November 2011

Publikationsdatum:
28. Dezember 2011 (online)

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Abstract

Aquaglyceroporin 7 (AQP7) is a glycerol transporter expressed in adipocytes. Its expression has been shown to be modulated in obesity. Metabolic syndrome is characterized by abdominal obesity, insulin resistance, dyslipidemia, and hypertension. An animal model displaying several features of metabolic syndrome was used to study the AQP7 expression at both mRNA and protein level and glycerol flux in adipocytes. Second generation n3-PUFA depleted female rats is a good animal model for metabolic syndrome as it displays characteristic features such as liver steatosis, visceral obesity, and insulin resistance. Our data show a reduced expression of AQP7 at the protein level in adipose tissue from n3-PUFA-depleted rats, without any changes at the mRNA levels. [U-14C]-Glycerol uptake was not modified in adipocytes from n3-PUFA-depleted animals.

Key words

aquaporins - fat cell - lipid metabolism
 

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