Planta Med 2011; 77 - PM198
DOI: 10.1055/s-0031-1282956

Simultaneous determination of four auxins in cyanobacterial extracts using HPLC-ESI-MS

M Seyed Hashtroudi 1, Z Shariatmadari 2, H Riahi 2, A Ghassempour 1
  • 1Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C. Evin Tehran, Iran
  • 2Faculty of Biosciences, Shahid Beheshti University, G.C. Evin, Tehran, Iran

The prokaryotic cyanobacteria are important source of structurally bioactive secondary metabolites[1]. Besides having considerable pharmacological impacts such as antibacterial, antifungal and also cytotoxicity, the potential biofertilizer activity of cyanobacteria make them an attractive alternative to chemical fertilizers. Most paddy soils have a natural population of cyanobacteria. Treatment of rice seedings with collected cyanobacteria from paddy fields of Iran showed positive growth effect in vitro [2] which could be partly attributed to the possible presence of phytohormons in their composition [3]. Herein, we report a new approach for the simultaneous determination of four important auxins including indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) with HPLC equipped with PDA detector at 220nm after extraction of the micoalgae using ultrasonic and microwave assisted extraction. The identities of the auxins were further confirmed using liquid

Chromatography-ESI-Mass spectrometry. Under the optimized conditions, a complete separation of four auxins was achieved within a short time with a good reproducibility.

The comparison of auxin chromatographic profile of this cyanobacteria with others and also their concentration levels will be reported and further discussed.

Keywords: Cyanobacteria, Auxin, HPLC-ESI-MS

References: 1- Tan LT (2007) Phytochem 68: 954–979

2- Saadatnia H, Riahi H (2009) Plant Soil Environ 55:207–212

3- Sergeeva E, Liaimer A, Bergman B (2002) Planta 215: 229–238