J Reconstr Microsurg 2011; 27(6): 331-336
DOI: 10.1055/s-0031-1278710
© Thieme Medical Publishers

An Optimized Method of Vessel Dissection in Establishment of the Rat Aortic Transplantation Model

Ming Luo1 , [*] , Feng Qiu2 , [*] , Jianxin Qiu1 , Yong Liu1 , Yu Fan1 , Yifeng Guo1
  • 1Department of Urology and Renal Transplantation, Affiliated First People's Hospital, Shanghai Jiao Tong University, Shanghai, China
  • 2Transplantation Center, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China
Further Information

Publication History

Publication Date:
24 May 2011 (online)

ABSTRACT

The high demand for microsurgical skills in those without a strong microsurgery background limits the application of the rat aortic transplant model to transplantation research. In this study, we established a rat aortic transplant model using a hydrodissection technique as a minimal-touch technique in vessel dissection. Eighty male Sprague Dawley rats were randomly divided into two groups with equal numbers. In the experimental group, abdominal aortas were harvested using hydrodissection; in the control group, instrumental dissection was used. The harvested aortas were transplanted orthotopically. The mean harvesting and implanting time in the experimental group was significantly lower than that of the control group (11.8 ± 1.51 versus 23.8 ± 3.38 minutes, p < 0.01; 46 ± 6.07 versus 58.85 ± 5.31 minutes, p < 0.01). Surgical complications in the control group included inferior vena cava injury (2/20), arterial vasospasm (3/20), and arterial wall hemorrhage (1/10). None of these complications were observed in the hydrodissection group. The overall frequency of surgical complications in the hydrodissection group was significantly lower than that in the control group (p < 0.05). This study suggested that the hydrodissection technique is a fast and safe method of vessel dissection. This technique requires less microsurgical skills and optimizes the establishment of the rat aortic transplant model.

REFERENCES

  • 1 Häyry P, Mennander A, Tiisala S, Halttunen J, Yilmaz S, Paavonen T. Rat aortic allografts: an experimental model for chronic transplant arteriosclerosis.  Transplant Proc. 1991;  23 (1 Pt 1) 611-612
  • 2 Mennander A, Tiisala S, Halttunen J, Yilmaz S, Paavonen T, Häyry P. Chronic rejection in rat aortic allografts. An experimental model for transplant arteriosclerosis.  Arterioscler Thromb. 1991;  11 671-680
  • 3 Onuta G, Rienstra H, de Boer J F et al.. Rosiglitazone attenuates transplant arteriosclerosis after allogeneic aorta transplantation in rats.  Transplantation. 2007;  84 517-526
  • 4 Shimizu H, Takahashi M, Takeda S et al.. Mycophenolate mofetil prevents transplant arteriosclerosis by direct inhibition of vascular smooth muscle cell proliferation.  Transplantation. 2004;  77 1661-1667
  • 5 Andriambeloson E, Pally C, Hengerer B et al.. Transplantation-induced endothelial dysfunction as studied in rat aorta allografts.  Transplantation. 2001;  72 1881-1889
  • 6 Blain B, Zhang F, Jones M et al.. Vascular grafts in the rat model: an anatomic study.  Microsurgery. 2001;  21 80-83
  • 7 Neil D A, Lynch S V, Hardie I R, Effeney D J. Endothelium during microarterial graft procurement and transplantation.  Microsurgery. 2000;  20 121-125
  • 8 Papachristou D N, Barters R. Resection of the liver with a water jet.  Br J Surg. 1982;  69 93-94
  • 9 Toth S, Vajda J, Pasztor E, Toth Z. Separation of the tumor and brain surface by “water jet” in cases of meningiomas.  J Neurooncol. 1987;  5 117-124
  • 10 Nagy L, Ishii K, Karatas A et al.. Water dissection technique of Toth for opening neurosurgical cleavage planes.  Surg Neurol. 2006;  65 38-41 discussion 41
  • 11 Piek J, Oertel J, Gaab M R. Waterjet dissection in neurosurgical procedures: clinical results in 35 patients.  J Neurosurg. 2002;  96 690-696
  • 12 Daniller A, Buchholz R, Chase R A. Renal transplantation in rats with the use of microsurgical techniques: a new method.  Surgery. 1968;  63 956-961
  • 13 Koulack J, McAlister V C, Giacomantonio C A, Bitter-Suermann H, MacDonald A S, Lee T D. Development of a mouse aortic transplant model of chronic rejection.  Microsurgery. 1995;  16 110-113
  • 14 Wollin M, Abele S, Bruns H et al.. Inhibition of TNF-alpha reduces transplant arteriosclerosis in a murine aortic transplant model.  Transpl Int. 2009;  22 342-349
  • 15 Vollmer C M, Dixon E, Sahajpal A et al.. Water-jet dissection for parenchymal division during hepatectomy.  HPB (Oxford). 2006;  8 377-385
  • 16 Kaehler G F, Sold M G, Fischer K, Post S, Enderle M. Selective fluid cushion in the submucosal layer by water jet: advantage for endoscopic mucosal resection.  Eur Surg Res. 2007;  39 93-97
  • 17 Shekarriz B. Hydro-Jet technology in urologic surgery.  Expert Rev Med Devices. 2005;  2 287-291
  • 18 Shekarriz B, Upadhyay J, Jewett M A. Nerve-sparing retroperitoneal lymphadenectomy using hydro-jet dissection: initial experience.  J Endourol. 2004;  18 273-276
  • 19 Tschan C, Gaab M R, Krauss J K, Oertel J. Waterjet dissection of the vestibulocochlear nerve: an experimental study.  J Neurosurg. 2009;  110 656-661
  • 20 Nakagawa A, Kumabe T, Kanamori M et al.. Clinical application of pulsed laser-induced liquid jet: preliminary report in glioma surgery.  No Shinkei Geka. 2008;  36 1005-1010

1 Luo and Qiu contributed equally to this work.

Jianxin QiuM.D. Ph.D. 

Department of Urology and Renal Transplantation, Affiliated First People's Hospital, Shanghai Jiao Tong University, No. 100

Haining Road, Shanghai 200080, China

Email: jasonqiu@medmail.com.cn

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