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DOI: 10.1055/s-0030-1256382
© Georg Thieme Verlag KG Stuttgart · New York
Low dose endoluminal photodynamic therapy improves murine T cell-mediated colitis
Publication History
submitted 30 September 2008
accepted after revision 31 January 2011
Publication Date:
27 May 2011 (online)
![](https://www.thieme-connect.de/media/endoscopy/201107/lookinside/thumbnails/10.1055-s-0030-1256382-1.jpg)
Background and study aims: Low dose photodynamic therapy (LDPDT) may modify the mucosal immune response and may thus provide a therapy for Crohn’s disease. We evaluated the efficacy and safety of this technique in a murine T cell-mediated colitis model.
Methods: The safety of LDPDT was first tested in BALB/c mice. Naïve T cells were used to induce colitis in mice with severe combined immunodeficiency, which were followed up endoscopically, and a murine endoscopic index of colitis (MEIC) was developed. The efficacy of LDPDT (10 J/cm2; delta-aminolevulinic acid, 15 mg/kg bodyweight) was then tested on mice with moderate colitis, while a disease control group received no treatment. The MEIC, weight, length, and histology of the colon, cytokine expression indices, number of mucosal CD4+ T cells, percentage of apoptotic CD4+ T cells, body weight, and systemic side effects were evaluated.
Results: LDPDT improved the MEIC (P = 0.011) and the histological score (P = 0.025), diminished the expression indices of the proinflammatory cytokines, interleukin-6 (P = 0.042), interleukin-17 (P = 0.029), and interferon-gamma (P = 0.014), decreased the number of mucosal CD4+ T cells, and increased the percentage of apoptotic CD4+ T cells compared with the disease control group. No local or systemic side effects occurred.
Conclusion: LDPDT improves murine T cell-mediated colitis, decreases the proinflammatory cytokines interleukin-6, interleukin-17, and interferon-gamma, and decreases the number of CD4+ T cells. No adverse events were observed. Therefore, this technique is now being evaluated in patients with inflammatory bowel disease.
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M.-A. OrtnerMD
Department of Gastroenterology, DMLL
University Hospital “Inselspital” Bern
Murtenstrasse
CH-3010 Bern
Germany
Fax: +41-31-6329765
Email: ma.ortner@bluewin.ch