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Int J Sports Med 2010; 31(8): 537-541
DOI: 10.1055/s-0030-1254115
Physiology & Biochemistry

© Georg Thieme Verlag KG Stuttgart · New York

Anti-Sports Anaemia Effects of Verbascoside and Martynoside in Mice

M. Zhu1 , 2 , N. Tan1 , H. Zhu3 , G. Zeng1 , W. He1 , B. Yu2 , X. Chen2
  • 1State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China
  • 2Institute of Sports Science, Zhanjiang Normal College, Zhanjiang, China
  • 3Institute of Sports Science, Huanan Normal University, Guangzhou, China
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Publication History

accepted after revision April 12, 2010

Publication Date:
16 June 2010 (online)

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Abstract

This paper aims to investigate the effects of verbascoside and martynoside isolated from Pedicularis dolichocymba on sports anaemia. Forty mice were divided into four groups: Group R (control group, nonsupplemented and maintained at rest), Group E (nonsupplemented and undergoing exercise), Group VE (supplemented with verbascoside 10 mg/kg per day and undergoing exercise), and Group ME (supplemented with martynoside 10 mg/kg per day and undergoing exercise). After 5 weeks intensive swimming exercises, we measured the RBC count, the hemoglobin concentration, the hematocrit (Hct), the mean corpuscular hemoglobin concentration (MCHC) and the mean corpuscular hemoglobin (MCH). We studied the shapes of RBC and measured the plasma malonyldialdehyde (MDA). We found Group E showed lower RBC, hemoglobin and Hct levels, higher MCHC, MCH, plasma MDA levels and the abnormally shaped RBCs percentage than Groups R, VE and ME. Group ME showed lower RBC and Hct levels, higher MCH, plasma MDA levels and the abnormally shaped RBCs percentage than Group VE. The results indicated that verbascoside and martynoside have the potential of antagonizing sports anaemia, the mechanism of this effect might be related to preventing RBC from free radical damage. Moreover, verbascoside was found to be more active than martynoside.

Key words

verbascoside - martynoside - anti-sports anaemia

References

  • 1 Amara F, Simpson L, Ghanem Y, Abdel-Aati T, Korani MA, Youssef E, El-Sheikh S. Study of erythrocyte shape changes in schistosomiasis.  Clin Hemorheol Microcirc. 2003;  28 21-28
    PubMedGoogle Scholar
  • 2 Beneke R, Bihn D, Hutler M, Leithauser RM. Haemolysis caused by alterations of α- and β-spectrin after 10–35 min of severe exercise.  Eur J Appl Physiol. 2005;  95 307-312
    CrossrefPubMedGoogle Scholar
  • 3 Boyadjiev N, Taralov Z. Red blood cell variables in highly trained pubescent athletes: a comparative analysis.  Br J Sports Med. 2000;  34 200-204
    CrossrefPubMedGoogle Scholar
  • 4 Chu HB, Tan NH, Xiong J, Zhang YM, Ji CJ. Chemical constituents of Pedicularis dolichocymba Hand.-Mazz.  Nat Prod Res Dev. 2007;  19 584-587
    PubMedGoogle Scholar
  • 5 Cooper CE, Vollaard NB, Choueiri T, Wilson MT. Exercise, free radicals and oxidative stress.  Biochem Soc Trans. 2002;  30 280-285
    CrossrefPubMedGoogle Scholar
  • 6 Di Santolo M, Stel G, Banfi G, Gonano F, Cauci S. Anemia and iron status in young fertile non-professional female athletes.  Eur J Appl Physiol. 2008;  102 703-709
    CrossrefPubMedGoogle Scholar
  • 7 Jia ZJ, Gao JJ, Liu ZM. Iriodid and phenylpropanoid glycosides from Pedicularis plicata Maxim.  Indian J Chem. 1994;  33B 460-464
    PubMedGoogle Scholar
  • 8 Jordan J, Kiernan W, Merker HJ, Wenzel M, Beneke R. Red cell membrane skeletal changes in marathon runners.  Int J Sports Med. 1998;  19 16-19
    Article in Thieme ConnectPubMedGoogle Scholar
  • 9 Harriss DJ, Atkinson G. International Journal of Sports Medicine – Ethical Standards in Sport and Exercise Science Research.  Int J Sports Med. 2009;  30 701-702
    Google Scholar
  • 10 Kocturk S, Kayatekin BM, Resmi H, Acikgoz O, Kaynak C, Ozer E. The apoptotic response to strenuous exercise of the gastrocnemius and solues muscle fibers in rats.  Eur J Appl Physiol. 2008;  102 515-524
    CrossrefPubMedGoogle Scholar
  • 11 Lang F, Lang KS, Lang PA, Huber SM, Wieder T. Mechanisms and significance of eryptosis.  Antioxid Redox Signal. 2006;  8 1183-1192
    CrossrefPubMedGoogle Scholar
  • 12 Li J, Zheng RL, Liu ZM, Jia ZJ. Scavenging effects of phenylpropanoid glycosides on superoxide and its antioxidative effects.  Acta Pharmacol Sin. 1992;  13 427-430
    PubMedGoogle Scholar
  • 13 Li JX, Xin D, Li H, Lu JF, Tong CW, Gao JN, Chan KM. Effect of verbascoside on decreasing concentration of oxygen free radicals and lipid peroxidation in skeletal muscle.  Acta Pharmacol Sin. 1999;  20 126-130
    PubMedGoogle Scholar
  • 14 Liao F, Zheng RL, Gao JJ, Jia ZJ. Retardation of skeletal muscle fatigue by the two phenylpropanoid glycosides: verbascoside and martynoside from Pedicularis plicata Maxim.  Phytother Res. 1999;  13 621-623
    CrossrefPubMedGoogle Scholar
  • 15 Liu MJ, Li JX, Guo HZ, Lee KM, Qin L, Chan KM. The effects of verbascoside on plasma lipid peroxidation level and erythrocyte membrane fluidity during immobilization in rabbits: a time course study.  Life Sci. 2003;  73 883-892
    CrossrefPubMedGoogle Scholar
  • 16 Liu QS, Wang JH, Cui J, Yang ZH, Du GH. A novel acute anemia model for pharmacological research in mice by compelled acute exercise.  Acta Pharmacol Sin. 2009;  30 1643-1647
    CrossrefPubMedGoogle Scholar
  • 17 Liu ZM, Jia ZJ. Phenylpropanoid and iridoid glycosides from Pedicularis striata.  Phytochemistry. 1991;  30 1341-1344
    CrossrefPubMedGoogle Scholar
  • 18 Pan L, Cui XR, Cui L, Li Y, Li ZB. Scanning electron microscopic observation of erythrocytes in hyperlipemia rats.  J N Bethune Univ Med Sci (in Chinese). 2001;  27 124-126
    PubMedGoogle Scholar
  • 19 Peeling P, Dawson B, Goodman C, Landers G, Trinder D. Athletic induced iron deficiency: new insights into the role of inflammation, cytokines and hormones.  Eur J Appl Physiol. 2008;  103 381-391
    CrossrefPubMedGoogle Scholar
  • 20 Robinson Y, Cristancho E, Böning D. Intravascular hemolysis and mean red blood cell age in athletes.  Med Sci Sports Exerc. 2006;  38 480-483
    CrossrefPubMedGoogle Scholar
  • 21 Santos-Silva A, Castro EM, Teixeira NA, Guerra FC, Quintanilha A. Erythrocyte membrane band 3 profile imposed by cellular aging, by activated neutrophils and by neutrophilic elastase.  Clin Chim Acta. 1998;  275 185-196
    CrossrefPubMedGoogle Scholar
  • 22 Santos-Silva A, Rebelo MI, Castro EM, Belo L, Guerra A, Rego C, Quintanilha A. Leukocyte activation, erythrocyte damage, lipid profile and oxidative stress imposed by high competition physical exercise in adolescents.  Clin Chim Acta. 2001;  306 119-126
    CrossrefPubMedGoogle Scholar
  • 23 Smith JA. Exercise, training and red blood cell turnover.  Sports Med. 1995;  19 9-31
    CrossrefPubMedGoogle Scholar
  • 24 Szygula Z. Erythrocytic system under the influence of physical exercise and training.  Sports Med. 1990;  10 181-197
    CrossrefPubMedGoogle Scholar
  • 25 Temiz A, Ba°kurt OK, Pekçetin C, Kandemir F, Güre A. Leukocyte activation, oxidant stress and red blood cell properties after acute, exhausting exercise in rats.  Clin Hemorheol Microcirc. 2000;  22 253-259
    PubMedGoogle Scholar
  • 26 Wang P, Kang J, Zheng R, Yang Z, Lu J, Gao J, Jia Z. Scavenging effects of phenylpropanoid glycosides from Pedicularis on superoxide anion and hydroxyl radical by the spin trapping method (95) 02255-4.  Biochem Pharmacol. 1996;  51 687-691
    CrossrefPubMedGoogle Scholar
  • 27 Weight LM, Byrne MJ, Jacobs P. Haemolytic effects of exercise.  Clin Sci (Lond). 1991;  81 147-152
    PubMedGoogle Scholar
  • 28 Yusof A, Leithauser RM, Roth HJ, Finkernagel H, Wilson MT, Beneke R. Exercise-induced hemolysis is caused by protein modification and most evident during the early phase of an ultraendurance race.  J Appl Physiol. 2007;  102 582-586
    CrossrefPubMedGoogle Scholar
  • 29 Zheng RL, Wang PF, Li J, Liu ZM, Jia ZJ. Inhibition of autoxidation of linoleic acid by phenylpropanoid glycosides from Pedicularis in micelles.  Chem Phys Lipids. 1993;  65 151-154
    CrossrefPubMedGoogle Scholar

Correspondence

Prof. Dr. Ninghua Tan

State Key Laboratory of

Phytochemistry and Plant

Resources in West China

Kunming Institute of Botany

Chinese Academy of Sciences

132# Lanhei Road, Heilongtan

Kunming 650204

China

Phone: +86/871/522 3800

Fax: +86/871/522 3800

Email: nhtan@mail.kib.ac.cn