The physiological status of the colon or ceacum is known to be very important for the host organism. Therefore, the aim of this study was to estimate the influence of high doses of polyphenolic extracts from chokeberry (CH), blue-berried honeysuckle (H), and green tea (GT) on fermentation processes in the caecum and caecal parameters of rats fed casein diets. In a 4-week experiment, 35-day-old rats were fed diets containing 0.4, 0.8, and 1.2 % of pure polyphenols. The greatest weight of digesta was recorded in rats fed 1.2 % of GT extract, and these animals were also characterised by having the lowest content of dry matter. Supplementation of diets with the extracts of interest caused a reduction in pH values and ammonia concentrations in caecal digesta in comparison to control animals. The results of a two-way analysis of variance indicated dose-dependent (except for 0.4 % supplementation) inhibition of enzymatic activity compared to control animals. Introduction of CH and H extracts significantly reduced the activity of β-glucuronidase compared to rats fed tea diets. Two-way analysis of variance showed a significant decrease in volatile fatty acids concentration in rats fed diets supplemented with H and CH extracts in comparison to control and tea-fed rats. The obtained results showed that the extracts tested can distinctly influence caecal parameters and metabolism.
Key words
polyphenols - extract - caecum - fermentation - rat
References
1
Kuroda Y, Hara Y.
Antimutagenic and anticarcinogenic activity of tea polyphenols.
Mutat Res.
1999;
436
69-97
3
Facino M R, Carini M, Aldini G, Berti F, Rossoni G, Bombardelli G, Morazzoni P.
Diet enriched with procyanidins enhances antioxidant activity and reduces myocardial post-ischaemic damage in rats.
Life Sci.
1999;
64
627-642
4
Mei Y, Wei D, Liu J.
Modulation effect of tea polyphenol toward N-methyl-N-nitro-N-nitrosoguanidine-induced precancerous gastric lesions in rats.
J Nutr Biochem.
2005;
16
172-177
9
Frejnagel S.
Comparison of polyphenolic composition of extracts from honeysuckle, chokeberries and green tea – a short report.
Pol J Food Nutr Sci.
2007;
57
83-86
11
Gonthier M-P, Verny M-A, Besson C, Remesey C.
Chlorogenic acid bioavailability largely depends on its metabolism by the gut microflora in rats.
J Nutr.
2003;
133
1853-1859
12
Simons A L, Renouf M, Hendrich S, Murphy P A.
Human gut microbial degradation of flavanoids: structure-function relationships.
J Agric Food Chem.
2005;
53
4258-4263
13
Selma M V, Espin J C, Tomas-Barberan F A.
Interaction between phenolics and gut microbiota: role in human health.
J Agric Food Chem.
2009;
57
6485-6501
14
Rechner A R, Smith M A, Kuhnle G, Gibson G R, Debnam E S, Srai S K S, Moore K P, Rice-Evans C A.
Colonic metabolism of dietary polyphenols: influence of structure on microbial fermentation products.
Free Radic Biol Med.
2004;
36
212-225
16
Aprikian O, Duclos V, Guyot S, Besson C, Manach C, Bernalier A, Morand C, Remesey C, Demigne C.
Apple pectin and a polyphenol-rich apple concentrate are more effective together than separately on cecal fermentation and plasma lipids in rats.
J Nutr.
2003;
133
1860-1865
18
Levrat M A, Texier O, Regerat F, Demigne C, Remesey C.
Comparison of the effects of condensed tannin and pectin on cecal fermentations and lipid metabolism in the rat.
Nutr Res.
1993;
13
427-433
21
Reddy B S, Engle A, Simi B, Goldman M.
Effect of dietary fiber on colonic bacterial enzymes and bile acids in relation to colon cancer.
Gastroenterology.
1992;
102
1475-1482
23
Reeves P.
AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformation of the AIN-76A rodent diet.
J Nutr.
1997;
123
1939-1951
24
Hofirek B, Haas D.
Comparative studies of ruminal fluid collected by oral tube or by puncture of the caudorental ruminal sac.
Acta Vet Brno.
2001;
70
27-33
25
Djouzi Z, Andrieux C.
Compared effect of three oligosaccharides on metabolism of intestinal microflora on rats inoculated with a human faecal flora.
Br J Nutr.
1997;
78
313-324
27
Kuhnle G, Spencer J P E, Schroeter H, Shenoy B, Debnam E S, Srai S K, Rice-Evans C, Hahn U.
Epicatechin and catechin are O-methylated and glucuronidated in the small intestine.
Biochem Biophys Res Commun.
2000;
277
507-512
28
Keppler K, Humpf H-U.
Metabolism of anthocyanins and their phenolic degradation products by the intestinal microflora.
Bioorg Med Chem.
2005;
13
5195-5205
29
Jansman A J M, Verstegen M W A, Huisman J.
Effects of dietary inclusion of hulls of faba beans (Vicia faba L.) with a low and high content of condensed tannins on digestion and some physiological parameters in piglets.
Anim Feed Sci Technol.
1993;
43
239-257
31
Bazzocco S, Mattila I, Guyot S, Renard C M, Aura A M.
Factors affecting the conversion of apple polyphenols to phenolic acids and fruit matrix to short-chain fatty acids by human faecal microbiota in vitro.
Eur J Nutr.
2008;
47
442-452
32
Deprez S, Brezillon C, Rabot S, Philippe C, Mila I, Lapierre C, Scalbert A.
Polymeric proanthocyanidins are catabolized by human colonic microflora into low-molecular-weight phenolic acids.
J Nutr.
2000;
130
2733-2738
33
Vitaglione P, Donnarumma G, Napolitano A, Galvano F, Gallo A, Scalfi L, Fogliano V.
Protocatechuic acid is the major human metabolite of cyaniding-glucosides.
J Nutr.
2007;
137
2043-2048
34
Bravo L, Abia R, Eastwood M A, Saura-Calixto F.
Degradation of polyphenols (catechin and tannic acid) in the rat intestinal tract. Effect on colonic fermentation and faecal output.
Br J Nutr.
1994;
71
933-946
36
Kim D-H, Jung E-A, Sohng I-S, Han J-A, Kim T-H, Han M J.
Intestinal bacterial metabolism of flavonoids and its relation to some biological activities.
Arch Pharm Res.
1998;
21
17-23
38
Mai V, Katki H A, Harmsen H, Gallaher D, Schatzkin A, Baer D J, Clevidence B.
Effects of a controlled diet and black tea drinking on the fecal microflora composition and the fecal bile acid profile of human volunteers in a double-blinded randomized feeding study.
J Nutr.
2004;
134
473-478