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Planta Med 2010; 76(17): 1987-1996
DOI: 10.1055/s-0030-1250520
Traditional Chinese Medicine
Reviews
© Georg Thieme Verlag KG Stuttgart · New York

Application of Mid-Infrared Spectroscopy in the Quality Control of Traditional Chinese Medicines

Suqin Sun1 , Jianbo Chen1 , Qun Zhou1 , Guanghua Lu2 , Kelvin Chan3
  • 1Key Laboratory of the Ministry of Education in China on Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, China
  • 2Key Laboratory of the Ministry of Education in China on the Standardization of Chinese Materia Medica, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China
  • 3Herbal Medicine Research & Education Centre, Faculty of Pharmacy, University of Sydney and Centre for Complementary Medicine Research, College of Health & Science, University of Western Sydney, Sydney, Australia
Weitere Informationen

Publikationsverlauf

received February 11, 2010 revised Sept. 29, 2010

accepted October 7, 2010

Publikationsdatum:
03. November 2010 (online)

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Abstract

Chinese herbal medicines are often referred to as Chinese materia medica (CMM). Composite formulae containing mixtures of CMM are prescribed for treatment and prevention of diseases in the practice of traditional Chinese medicine (TCM). Some of the well-known CMM formulae (Fufang in Chinese) are manufactured and marketed as proprietary Chinese medicines (PCM). Quality assessment and assurance of these products are difficult; they are a challenging task. Mid-infrared spectroscopy, a classic molecular structure analysis method, has been innovatively applied in the quality control of TCM, and has gained significant impact and advancement in analytical fields. Infrared fingerprinting features appear particularly suitable for the identification of multicomponent matrices in samples whose chemical integrity has not been altered or destroyed because no extraction procedure is needed. This review summarizes and gives an overall view on the application of mid-infrared and two-dimensional correlation infrared (2D‐IR) spectroscopy as well as chemometric techniques in the identification of CMM, investigation of TCM processing procedures, and analysis of herb extracts and preparations.

Key words

traditional Chinese medicine - quality control - mid‐infrared spectroscopy - two‐dimensional correlation infrared spectroscopy - chemometric techniques

References

  • 1 Liang Y Z, Xie P S, Chan K. Quality control of herbal medicines.  J Chromatogr B. 2004;  812 53-70
    CrossrefPubMedGoogle Scholar
  • 2 Sun S Q, Zhang X, Qin Z, Hu X Y. Direct determination of raw plant drugs by FTIR.  Spectrosc Spectral Anal. 1999;  19 542-545
    PubMedGoogle Scholar
  • 3 Sun S Q, Yu J Y, Hu X Y. New researches in the direct determination of Chinese traditional drugs with molecular spectroscopy.  Spectrosc Spectral Anal. 1999;  19 841-843
    PubMedGoogle Scholar
  • 4 Sun S Q, Zhou Q, Yu J Y, Hu X Y. The latest development of the research on Chinese medicine by molecular vibrational spectroscopy.  Spectrosc Spectral Anal. 2000;  20 199-202
    PubMedGoogle Scholar
  • 5 Liu H, Sun S Q. Application and progress of molecular vibrational spectrum in traditional Chinese medicine modernization.  Med Instrum. 2005;  5 6-10
    PubMedGoogle Scholar
  • 6 Sun S Q, Zhou Q, Qin Z. Atlas of two-dimensional correlation infrared spectroscopy for traditional Chinese medicine identification. Beijing; Chemical Industry Press 2003
    Google Scholar
  • 7 Noda I. Two-dimensional infrared spectroscopy of synthetic and biopolymers.  Bull Am Phys Soc. 1986;  31 520
    PubMedGoogle Scholar
  • 8 Noda I. Generalized two-dimensional infrared spectroscopy.  J Am Chem Soc. 1989;  111 8116-8118
    CrossrefPubMedGoogle Scholar
  • 9 Noda I. Generalized two-dimensional correlation method applicable to infrared, Raman, and other types of spectroscopy.  Appl Spectrosc. 1993;  47 1329-1336
    CrossrefPubMedGoogle Scholar
  • 10 Li Y M, Sun S Q, Zhou Q, Tao J X, Noda I. Study of traditional Chinese animal drugs using FT-IR and 2D-IR correlation spectroscopy.  Spectrochim Acta A Mol Biomol Spectrosc. 2006;  63 565-573
    CrossrefPubMedGoogle Scholar
  • 11 Wang L Q, Li Y M, Zhang L P, Xiao H X, Zhou Q, Sun S Q. Analysis and characterization of fingerprint infrared spectra for ecology-planted Coptis chinensis.  Spectrosc Spectral Anal. 2006;  26 1061-1066
    PubMedGoogle Scholar
  • 12 Pei L K, Sun S Q, Guo B L, Huang W H, Xiao P G. Fast quality control of Herba Epimedii by using Fourier transform infrared spectroscopy.  Spectrochim Acta A Mol Biomol Spectrosc. 2008;  70 258-264
    CrossrefPubMedGoogle Scholar
  • 13 Cheung Y M, Zhou Q, Guo B L, Sun S Q. Identifying the characteristics of FTIR spectra of Herba Epimedii Icariin via wavelet analysis and RBF neural network.  Spectrosc Spectral Anal. 2009;  29 1830-1834
    PubMedGoogle Scholar
  • 14 Cao F, Zhou Q, Sun S Q. Study on the identification of standard and false Tianma by two-dimensional infrared correlation spectroscopy.  Med Instrum. 2002;  4 19-21
    PubMedGoogle Scholar
  • 15 Zhou Q, Li J, Liu J, Huang H, Sun S Q. Two-dimensional correlation infrared spectroscopy of standard and false Dahuang.  Chin J Anal Chem. 2003;  31 1058-1061
    PubMedGoogle Scholar
  • 16 Sun S Q, Zhou Q, Liu J, Huang H. Study on the identification of standard and false Banxia by two-dimensional infrared correlation spectroscopy.  Spectrosc Spectral Anal. 2004;  24 427-430
    PubMedGoogle Scholar
  • 17 Xu C H, Zhou Q, Sun S Q, Wang B Q. The identification of Ejiao by two dimensional correlation infrared spectroscopy.  Chin J Anal Chem. 2005;  33 221-224
    PubMedGoogle Scholar
  • 18 Ayiguli T, Zhou Q, Dong X O, Sun S Q. Study on the identification of standard and false Gancao by Fourier transform infrared spectroscopy.  Spectrosc Spectral Anal. 2006;  26 1238-1241
    PubMedGoogle Scholar
  • 19 Zhou J, Sun J Y, Xu S Y, Zhou Q, Sun S Q. Study on the identification of Illicium vatum Hook. f. and Illicium lanceolatum A. C. Smith by multi-steps infrared macro-fingerprint method.  Spectrosc Spectral Anal. 2008;  28 2864-2867
    PubMedGoogle Scholar
  • 20 Huang D L, Sun S Q, Xu Y Q, Chen X K. Analysis and identification of Dangshen and its counterfeit Yeguanmen by Fourier transform infrared spectroscopy.  Med Instrum. 2008;  5 22-25
    PubMedGoogle Scholar
  • 21 Cai F, Sun S Q, Yan W R, Niu S J, Li X E. Identification and analysis of genuine and false Flos Rosae Rugosae by FTIR and 2D correlation IR spectroscopy.  Spectrosc Spectral Anal. 2009;  29 2429-2433
    PubMedGoogle Scholar
  • 22 Chen J, Sun S Q, Xu R, Liu Y G, Yu J, Liu T N, Li J Q. Identification of Cistanche deserticola from Boschniakla rossica and Cynomorium songaricum using FTIR and two-dimensional correlation IR spectroscopy.  Spectrosc Spectral Anal. 2009;  29 1502-1507
    PubMedGoogle Scholar
  • 23 Yang P, Song P, Sun S Q, Zhou Q, Feng S, Tao J X. Differentiation and quality estimation of Cordyceps with infrared spectroscopy.  Spectrochim Acta A Mol Biomol Spectrosc. 2009;  74 983-990
    CrossrefPubMedGoogle Scholar
  • 24 Cheng Z F, Xu R, Cheng C G. Study on identification of Gastrodia elata BI. by Fourier self-deconvolution infrared spectroscopy.  Spectrosc Spectral Anal. 2007;  27 1719-1722
    PubMedGoogle Scholar
  • 25 Cheng C G, Ying T K. Quality control of Corydalis yanhusuo W. T. Wang by second derivative FTIR spectroscopy combined with statistics.  Spectrosc Spectral Anal. 2005;  25 36-38
    PubMedGoogle Scholar
  • 26 Cheng C G, Sun C R, Pan Y J. Direct identification of Ophiogon japonicus (Thunb.)Ker-Gawl. from its confusable varieties by second derivative FTIR spectroscopy combined with statistics.  Spectrosc Spectral Anal. 2004;  24 1055-1059
    PubMedGoogle Scholar
  • 27 Jin W Y, Cheng C G, Wu X H. Identification of Rhizoma Atractylodes based on FTIR spectra and radial basis function network.  Spectrosc Spectral Anal. 2006;  26 2210-2213
    PubMedGoogle Scholar
  • 28 Ma S M, Liu S D, Zhang Z Y, Fan G Q. Radial basis function networks and IR spectrometry applied for identification of official Rhubarb samples.  Spectrosc Spectral Anal. 2005;  25 874-877
    PubMedGoogle Scholar
  • 29 Han M X, Zhou Q, Li Q H, Sun S Q. Identification of three-step IR spectra of Radix Puerariae from different habitats.  Spectrosc Spectral Anal. 2009;  29 1851-1855
    PubMedGoogle Scholar
  • 30 Hua R, Sun S Q, Zhou Q, Noda I, Wang B Q. Discrimination of Fritillary according to geographical origin with Fourier transform infrared spectroscopy and two-dimensional correlation IR spectroscopy.  J Pharm Biomed Anal. 2003;  33 199-209
    CrossrefPubMedGoogle Scholar
  • 31 Li Y M, Sun S Q, Zhou Q, Qin Z, Tao J X, Wang J, Fang X. Identification of American ginseng from different regions using FT-IR and two-dimensional correlation IR spectroscopy.  Vib Spectrosc. 2004;  36 227-232
    CrossrefPubMedGoogle Scholar
  • 32 Zhou X, Sun S Q, Huang Q H. Identification of Pericarpium Citri Reticulatae from different regions using FTIR.  Spectrosc Spectral Anal. 2007;  27 2453-2455
    PubMedGoogle Scholar
  • 33 Sun S Q, Yuan Z M, Bai Y. Identify the genuine of Shanyao (Dioscorea opposite Thunb.) by FTIR combined with computer aided analysis.  Comput Appl Chem. 2002;  19 77-80
    PubMedGoogle Scholar
  • 34 Xu Y Q, Sun S Q, Yuan Z M, Bai Y. Discrimination of trueborn tuber dioscoreae by fingerprint infrared spectra and principal component analysis.  Chin J Anal Chem. 2002;  30 1231-1233
    PubMedGoogle Scholar
  • 35 Sun S Q, Tang J M, Yuan Z M, Bai Y. FTIR and classification study on trueborn tuber dioscoreae samples.  Spectrosc Spectral Anal. 2003;  23 258-261
    PubMedGoogle Scholar
  • 36 Zhou Q, Sun S Q, Leung H W. FTIR and classification study on Gouqi from different cultural areas.  Spectrosc Spectral Anal. 2003;  23 509-511
    PubMedGoogle Scholar
  • 37 Liu S H, Zhang X G, Zhou Q, Sun S Q. Use of FTIR and pattern recognition to determine geographical origins of Chinese medical herbs.  Spectrosc Spectral Anal. 2005;  25 878-881
    PubMedGoogle Scholar
  • 38 Xu Y Q, Sun S Q, Feng X F, Hu S L. Quick identification of Skullcaps in different geographical origins using clustering analysis method and infrared fingerprint spectra.  Spectrosc Spectral Anal. 2003;  23 502-505
    PubMedGoogle Scholar
  • 39 Xu Y Q, Huang H, Zhou Q, Zhou H T, Hu S L, Sun S Q. The application of fingerprint infrared spectra and clustering analysis in the discrimination of geographical origin of Paeonia lactiflora Pall.  Chin J Anal Chem. 2003;  31 5-9
    PubMedGoogle Scholar
  • 40 Wang F L, Zhou J K, Wu J, Yu L, Sun S Q. Study on the identification of cultivated and wilding Danshen by multi-steps infrared macro-fingerprint method.  Med Instrum. 2006;  5 18-20
    PubMedGoogle Scholar
  • 41 Liu D, Li Y G, Xu H, Sun S Q, Wang Z T. Differentiation of the root of Cultivated Ginseng, Mountain Cultivated Ginseng and Mountain Wild Ginseng using FT-IR and two-dimensional correlation IR spectroscopy.  J Mol Struct. 2008;  883 228-235
    CrossrefPubMedGoogle Scholar
  • 42 Liu G, Dong Q, Yu F, Liu J H, Sun S Z. Identification of Gastrodia elata Blume by Fourier transform infrared spectroscopy.  Spectrosc Spectral Anal. 2004;  24 308-310
    PubMedGoogle Scholar
  • 43 Dong B, Sun S Q, Zhou H T, Hu S L. Rapid and undamaged determination of Chishao by Fourier-transform infrared spectroscopy and clustering analysis.  Spectrosc Spectral Anal. 2002;  22 232-234
    PubMedGoogle Scholar
  • 44 Dong B, Sun S Q, Qin Z, Zhou H T, Hu S L. Rapid and undamaged determination of Chishao by Fourier-transform infrared spectroscopy and clustering analysis.  Comput Appl Chem. 2002;  19 89-92
    PubMedGoogle Scholar
  • 45 Xu R, Sun S Q, Liu Y G, Yu J, Zhou F, Liu T N, Chen J, Chen S L. Rapid and undamaged determination of Cistanche deserticola by Fourier-transform infrared spectroscopy and clustering analysis.  Spectrosc Spectral Anal. 2009;  29 1860-1863
    PubMedGoogle Scholar
  • 46 Xu Y Q, Zhou Q, Sun S Q, Cai S Q. Recognition of three classes of Skullcaps by FTIR spectroscopy combined with artificial neural networks.  Spectrosc Spectral Anal. 2002;  22 945-948
    PubMedGoogle Scholar
  • 47 Huang H, Sun S Q, Xu J W, Wang Z. Novel application of FTIR in medical herb chemotaxonomy.  Spectrosc Spectral Anal. 2003;  23 253-257
    PubMedGoogle Scholar
  • 48 Lu G H, Zhou Q, Sun S Q, Leung K S Y, Zhang H, Zhao Z Z. Differentiation of Asian ginseng, American ginseng and Notoginseng by Fourier transform infrared spectroscopy combined with two-dimensional correlation infrared spectroscopy.  J Mol Struct. 2008;  883 91-98
    CrossrefPubMedGoogle Scholar
  • 49 Wang Z, Sun S Q, Li X B, Zhou Q, Lin L, Du D G. Direct determination of Rhizoma Cimicifuga by FTIR spectroscopy.  Spectrosc Spectral Anal. 2001;  21 311-313
    PubMedGoogle Scholar
  • 50 Peng Y, Sun S Q, Zhao Z Z, Leung H W. A rapid method for identification of genus Lycium by FTIR spectroscopy.  Spectrosc Spectral Anal. 2004;  24 679-681
    PubMedGoogle Scholar
  • 51 Pei L K, Guo B L, Sun S Q, Huang W H. Study on the identification of some species of Herba Epimedii with FTIR.  Spectrosc Spectral Anal. 2008;  28 55-60
    PubMedGoogle Scholar
  • 52 Chen J B, Zhou Q, Noda I, Sun S Q. Quantitative classification of two-dimensional correlation spectra.  Appl Spectrosc. 2009;  63 920-925
    CrossrefPubMedGoogle Scholar
  • 53 Chen J B, Zhou Q, Noda I, Sun S Q. Discrimination of different genera Astragalus samples via quantitative symmetry analysis of two-dimensional hetero correlation spectra.  Anal Chim Acta. 2009;  649 106-110
    CrossrefPubMedGoogle Scholar
  • 54 Lu G H, Sun S Q, Leung H W, Chan K K C. Study on the identification of Dangguitou and Dangguiwei by two-dimensional infrared correlation spectroscopy.  Spectrosc Spectral Anal. 2004;  24 311-314
    PubMedGoogle Scholar
  • 55 Jin Z X, Xu S Y, Sun S Q, Zhou Q. Analysis of Acanthopanax senticosus Harms for different parts using Fourier transform infrared spectroscopy.  Spectrosc Spectral Anal. 2008;  28 2859-2863
    PubMedGoogle Scholar
  • 56 Xu R, Sun S Q, Liu Y G, Chen J, Chen S L, Zhou F. Analysis and evaluation of different radial part of Cistanche deserticola by Fourier transform infrared spectroscopy and two-dimensional infrared correlation spectroscopy.  Chin J Anal Chem. 2009;  37 221-226
    PubMedGoogle Scholar
  • 57 Hong Q H, Cheng Z F, Cheng C G. Application of FTIR spectroscopy to the analysis of quality mensuration of Paeonia lactiflora Pall. from native habitat.  Spectrosc Spectral Anal. 2006;  26 1610-1613
    PubMedGoogle Scholar
  • 58 Zhan D Q, Cheung Y M, Sun S Q. Wavelet-transform based identification of ginseng of different ages using two-dimensional infrared correlation spectroscopy.  Spectrosc Spectral Anal. 2007;  27 1497-1501
    PubMedGoogle Scholar
  • 59 Zhou X, Sun S Q, Huang Q H. Identification of Pericarpium Citri Reticulatae from different years using FTIR.  Spectrosc Spectral Anal. 2008;  28 72-74
    PubMedGoogle Scholar
  • 60 Sun S Q, Wang M Q, Leung H W, Yeung H W. A rapid method for the identification of four different features of Gouqizi by FTIR spectrometry.  Spectrosc Spectral Anal. 2001;  21 787-789
    PubMedGoogle Scholar
  • 61 Liu Y, Wang J Q, Liu S H, Sun S Q. Two-dimensional correlation infrared spectroscopy applied to analyzing and identifying the Radix Paeoniae Alba medicinal materials.  J Mol Struct. 2008;  883 137-141
    CrossrefPubMedGoogle Scholar
  • 62 Yu L, Sun S Q, Fan K F, Zhou Q, Noda I. Research on processing medicinal herbs with multi-steps infrared macro-fingerprint method.  Spectrochim Acta A Mol Biomol Spectrosc. 2005;  62 22-29
    CrossrefPubMedGoogle Scholar
  • 63 Yu L, Sun S Q, Zhou Q, Qin Z. Research on parching procedure of white mustard seed with Fourier transform infrared spectroscopy and two-dimensional IR correlation spectroscopy.  Spectrosc Spectral Anal. 2006;  26 2181-2185
    PubMedGoogle Scholar
  • 64 Tang J M, Xian D, Sun S Q. Study on Radix Aconiti and Radix Aconiti Preparata by two-dimensional infrared correlation spectroscopy.  Med Instrum. 2005;  3 27-30
    PubMedGoogle Scholar
  • 65 Wang C L, Wen J M, Cheng T, Jiang K W, Sun S Q, Tu Y. Study on Aconitum kusnezoffii Reichb. and its processed products by 2D-IR correlation spectroscopy.  Spectrosc Spectral Anal. 2009;  29 1498-1501
    PubMedGoogle Scholar
  • 66 Sun S Q, Zhou Q, Leung H W. Study on Paofupian, Heishunpian and Baifupian by two-dimensional infrared correlation spectroscopy.  Spectrosc Spectral Anal. 2003;  23 1082-1085
    PubMedGoogle Scholar
  • 67 Bao H J, An H, Bai Y, Sun S Q. The analysis and identification of Chrysanthemum and processed Chrysanthemum by IR and second-order derivative spectroscopy.  Med Instrum. 2004;  5 26-28
    PubMedGoogle Scholar
  • 68 Xu C H, Sun S Q, Guo C Q, Zhou Q, Tao J X, Noda I. Multi-steps infrared macro-fingerprint analysis for thermal processing of Fructus Viticis.  Vib Spectrosc. 2006;  41 118-125
    CrossrefPubMedGoogle Scholar
  • 69 Liu H X, Sun S Q, Lv G H, Chan K K C. Study on Angelica and its different extracts by Fourier transform infrared spectroscopy and two-dimensional correlation IR spectroscopy.  Spectrochim Acta A Mol Biomol Spectrosc. 2006;  64 321-326
    CrossrefPubMedGoogle Scholar
  • 70 Liu H X, Sun S Q, Lv G H, Liang X Y. Discrimination of extracted lipophilic constituents of Angelica with multi-steps infrared macro-fingerprint method.  Vib Spectrosc. 2006;  40 202-208
    CrossrefPubMedGoogle Scholar
  • 71 Liu H X, Zhou Q, Sun S Q, Bao H J. Discrimination of different Chrysanthemum with Fourier transform infrared spectroscopy.  J Mol Struct. 2008;  883 38-47
    CrossrefPubMedGoogle Scholar
  • 72 Wu Y W, Xiao X H, Sun S Q, Liu H X. Study on traditional Chinese medicine extracts of Rhizoma Coptidis by FTIR spectroscopy.  Spectrosc Spectral Anal. 2009;  29 93-96
    PubMedGoogle Scholar
  • 73 Huang H, Jing L, Qin Z, Zhou Q, Sun S Q. The application of Fourier transform infrared spectroscopy on the quality control of traditional Chinese medicine formula particles.  Chin J Anal Chem. 2003;  31 828-832
    PubMedGoogle Scholar
  • 74 Zhou Q, Li J, Sun S Q, Liang X Y. Rapid identification of traditional Chinese medicine formula particles by Fourier transform infrared spectroscopy.  Chin J Anal Chem. 2003;  31 292-295
    PubMedGoogle Scholar
  • 75 Tang J M, Sun S Q, Yuan Z M, Qin Z. Study on the rapid and nondestructive identification of TCM formula particles by FTIR.  Spectrosc Spectral Anal. 2004;  24 554-556
    PubMedGoogle Scholar
  • 76 Wu J, Sun S Q, Zhou Q, Yu L. Study on rapid and nondestructive analysis of Danshen formula particles by FTIR.  Spectrosc Spectral Anal. 2007;  27 1535-1538
    PubMedGoogle Scholar
  • 77 Zuo L, Sun S Q, Tang J M. Two-dimensional correlation analysis of FT-IR spectra for research on the thermal stability of the Qingkailing injection.  Comput Appl Chem. 2002;  19 95-98
    PubMedGoogle Scholar
  • 78 Zuo L, Sun S Q, Zhou Q, Tao J X, Noda I. 2D-IR correlation analysis of deteriorative process of traditional Chinese medicine ‘Qing Kai Ling injection.  J Pharm Biomed Anal. 2003;  30 1491-1498
    CrossrefPubMedGoogle Scholar
  • 79 Zhou Q, Sun S Q, Zuo L. Study on traditional Chinese medicine ‘Qing Kai Ling injections from different manufactures by 2D IR correlation spectroscopy.  Vib Spectrosc. 2004;  36 207-212
    CrossrefPubMedGoogle Scholar
  • 80 Chen J B, Zhou Q, Sun S Q, Yu L, Xu K Y. Study on quality control of traditional Chinese medicine ginseng injection with Fourier transform infrared spectroscopy.  Spectrosc Spectral Anal. 2007;  27 1493-1496
    PubMedGoogle Scholar
  • 81 Zhang P, Sun S Q, Zhou Q, Xu K Y. Study on quality control of safflower injection and wild chrysanthemum injection with Fourier transform infrared spectroscopy.  Spectrosc Spectral Anal. 2007;  27 1539-1542
    PubMedGoogle Scholar
  • 82 Yan S, Xu M L, Tu Y, Li H F, Sun S Q. Qualitative and quantitative analysis of Shuanghuanglian fenzhenji by FTIR.  Spectrosc Spectral Anal. 2009;  29 1558-1561
    PubMedGoogle Scholar
  • 83 Wu Y W, Sun S Q, Zhou Q, Tao J X, Noda I. Volatility-dependent 2D IR correlation analysis of traditional Chinese medicine ‘Red Flower Oil preparation from different manufacturers.  J Mol Struct. 2008;  882 107-115
    CrossrefPubMedGoogle Scholar
  • 84 Wu Y W, Sun S Q, Zhou Q, Leung H W. Fourier transform mid-infrared (MIR) and near-infrared (NIR) spectroscopy for rapid quality assessment of Chinese medicine preparation Honghua Oil.  J Pharm Biomed Anal. 2008;  46 498-504
    CrossrefPubMedGoogle Scholar
  • 85 Chan K. Chinese medicinal materials and their interface with Western medical concepts.  J Ethnopharmacol. 2005;  96 1-18
    CrossrefPubMedGoogle Scholar

Prof. Suqin Sun

Department of Chemistry
Tsinghua University

Beijing 100084

China

Telefon: +86 10 62 78 76 61

Fax: +86 10 62 78 24 85

eMail: sunsq@mail.chem.tsinghua.edu.cn

Prof. Guanghua Lu

School of Pharmacy
Chengdu University of Traditional Chinese Medicine

Chengdu 611137

China

Telefon: +86 28 61 80 02 31

eMail: luguanghua@hotmail.com