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DOI: 10.1055/s-0030-1250477
© Georg Thieme Verlag KG Stuttgart · New York
Anti-Inflammatory and Anti-Melanogenic Steroidal Saponin Glycosides from Fenugreek (Trigonella foenum-graecum L.) Seeds
Publikationsverlauf
received April 6, 2010
revised October 1, 2010
accepted October 4, 2010
Publikationsdatum:
26. Oktober 2010 (online)
![](https://www.thieme-connect.de/media/plantamedica/201107/lookinside/thumbnails/10.1055-s-0030-1250477-1.jpg)
Abstract
Fenugreek seed (Trigonella foenum-graecum L.) is used as an herbal medicine for treating metabolic and nutritive dysfunctions. To determine if this plant has other beneficial effects, we tested the inhibitory activities of a methanol (MeOH) extract of fenugreek seed on the production of inflammatory cytokines and melanin synthesis in cultured cell lines in vitro. The MeOH extract inhibited the production of phorbol-12-myristate-13-acetate-induced inflammatory cytokines such as tumor necrosis factor (TNF)-α in cultured THP-1 cells, and also restrained the intracellular synthesis of melanin in murine melanoma B16F1 cells. We isolated three active constituents from fenugreek seed extracts. These were identified as the steroidal saponins 26-O-β-D-glucopyranosyl-(25R)-furost-5(6)-en-3β,22β,26-triol-3-O-α-L-rhamno-pyranosyl-(1′′→ 2′)-O-[β-D-glucopyranosyl-(1′′′ → 6′)-O]-β-D-glucopyranoside 1, minutoside B 2, and pseudoprotodioscin 3. Compounds 1 and 2 strongly suppressed the production of inflammatory cytokines, whereas 3 showed a weaker suppressing effect. Melanogenesis in B16F1 cells was significantly suppressed by 1 and 3, and weakly suppressed by 2. All three compounds showed moderate cytotoxicities. These results indicate that fenugreek extract and its active constituents could protect against skin damage.
Key words
Trigonella foenum‐graecum - Fabaceae - anti‐inflammatory - melanogenesis - steroidal saponin
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Associate Prof. Dr. Fumihide Takano
Pharmacognosy and Chemistry of Natural Products
School of Pharmacy and Pharmaceutical Sciences
Kanazawa University
Kakuma-machi
Kanazawa 920-1192
Japan
Telefon: +81 7 62 64 63 05
Fax: +81 7 62 64 62 41
eMail: takano@p.kanazawa-u.ac.jp
Prof. Dr. Tomihisa Ohta
Pharmacognosy and Chemistry of Natural Products
School of Pharmacy and Pharmaceutical Sciences
Kanazawa University
Kakuma-machi
Kanazawa 920-1192
Japan
Telefon: +81 7 62 34 44 68
Fax: +81 7 62 64 62 41
eMail: ohta@p.kanazawa-u.ac.jp
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