Planta Med 2011; 77(5): 461-466
DOI: 10.1055/s-0030-1250407
Natural Product Chemistry
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Protoflavonoids from Ferns Impair Centrosomal Integrity of Tumor Cells

Isabelle Pouny1 , Chantal Etiévant2 , Laurence Marcourt1 , Isabelle Huc-Dumas1 , Muriel Batut1 , Frédéric Girard2 , Michel Wright2 , Georges Massiot1
  • 1Unité Mixte de Service 2597, Centre National de la Recherche Scientifique (CNRS) – Pierre Fabre,, Institut des Sciences et Technologies du Médicament de Toulouse (ISTMT), Toulouse, France
  • 2Unité Mixte de Recherche 2587, Centre National de la Recherche Scientifique (CNRS) – Pierre Fabre, Institut des Sciences et Technologies du Médicament de Toulouse (ISTMT), Toulouse, France
Further Information

Publication History

received June 17, 2010 revised Sept. 10, 2010

accepted Sept. 15, 2010

Publication Date:
13 October 2010 (online)

Abstract

Six protoflavonoids, including two new compounds, were isolated during a large scale screening of fern extracts for original interaction with mitosis. The new compounds isolated from Phegopteris decursive-pinnata and Equisetum fluviatile were 2′,3′-dihydroprotogenkwanone (1) and 2′,3′-dihydro-2′-hydroxyprotoapigenone (2). Known compounds were: protoapigenone, protogenkwanone, protoapigenin, and 4′-O-β-D-glucopyranosyl protoapigenin. They showed a cytotoxic activity against HeLa cells at a micromolar level. IC50 values were 2 µM for compound 1, > 10 µM for compound 2, and respectively 2.4, 0.6, > 10 µM for the known compounds. Their cytotoxic effects were associated with phenotypic changes never observed before and characterized by the loss of centrosomal γ-tubulin labelling in both mitotic and interphasic cells.

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Dr. Georges Massiot

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