Planta Med 2011; 77(5): 434-440
DOI: 10.1055/s-0030-1250402
Biological and Pharmacological Activity
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Isolation of 5-(Hydroxymethyl)Furfural from Lycium chinense and its Inhibitory Effect on the Chemical Mediator Release by Basophilic Cells

Parida Yamada1 , Mai Nemoto2 , Hideyuki Shigemori2 , Shinichi Yokota3 , Hiroko Isoda1 , 2
  • 1Alliance for Research on North Africa, University of Tsukuba, Tsukuba, Japan
  • 2Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
  • 3Frontier Biochemical & Medical Research Laboratories, Corporate Research & Development Division, KANEKA Corporation, Takasago, Japan
Further Information

Publication History

received Dec. 7, 2009 revised August 2, 2010

accepted Sept. 11, 2010

Publication Date:
28 October 2010 (online)

Abstract

The effect of hot water extracts of Lycium chinense fruits (LCF) on the β-hexosaminidase (β-hexo) release by IgE sensitized BSA stimulated rat basophilic leukemia (RBL-2H3) cells was investigated. The ethylacetate (EtOAc) layer of the extract has shown an inhibitory effect on β-hexo release from RBL-2H3 cells at the antigen antibody binding stage. The water (H2O) fraction (EFW) of the chloroform (CHCl3) extract from the EtOAc layer also inhibited β-hexo release at the same stage in a dose-dependent manner. With column chromatography preparation, proton and carbon nuclear magnetic resonance (1H and 13C NMR) spectra, electron ionization mass spectrometer (EI‐MS) spectra, and high-performance liquid chromatography (HPLC) analysis, the active component was determined to be 5-(hydroxymethyl)furfural (5-HMF). Thus, the 5-HMF showed an inhibitory effect on β-hexo release at the antigen-antibody binding stage and the antibody-receptor binding stage. Furthermore, 5-HMF suppressed [Ca2+]i influx in the IgE-sensitized BSA-stimulated RBL-2H3 cells. Our results show that 5-HMF may be useful for the treatment or prevention of type I allergic diseases.

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Prof. Dr. Hiroko Isoda

Graduate School of Life and Environmental Sciences
University of Tsukuba

1-1-1 Tennodai, Tsukuba

Ibaraki 305-8572

Japan

Phone: +81 29 8 53 57 75

Fax: +81 29 8 53 57 76

Email: isoda@sakura.cc.tsukuba.ac.jp