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DOI: 10.1055/s-0030-1250383
© Georg Thieme Verlag KG Stuttgart · New York
Inhibitors of Nitric Oxide Production from Stemona javanica
Publication History
received May 1, 2010
revised July 22, 2010
accepted September 1, 2010
Publication Date:
01 October 2010 (online)
![](https://www.thieme-connect.de/media/plantamedica/201103/lookinside/thumbnails/10.1055-s-0030-1250383-1.jpg)
Abstract
In our screening program for bioactive natural products from our library of tropical plants, the extract prepared from the roots of Stemona javanica inhibited NO production in mouse macrophage-like cell line J774.1 stimulated by lipopolysaccharide (LPS). Bioassay-guided fractionation of the extract from S. javanica led to the isolation of two active compounds, stemofoline (1) and stemanthrene C (2). The inhibition mechanism of 1 was proposed to suppress iNOS expression in J774.1 cells stimulated by LPS, whereas that of 2 was due to potent radical scavenging activity resulting in NO inhibitory activity.
Key words
Stemona javanica - Stemonaceae - stemofoline - stemanthrene C - iNOS - DPPH radical scavenge
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Prof. Dr. Hiroshi Morita
Department of Pharmacognosy
Faculty of Pharmaceutical Sciences
Hoshi University
Shinagawa
Tokyo 142-8501
Japan
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Email: moritah@hoshi.ac.jp
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