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DOI: 10.1055/s-0030-1250268
© Georg Thieme Verlag KG Stuttgart · New York
Osthole Ameliorates Insulin Resistance by Increment of Adiponectin Release in High-Fat and High-Sucrose-Induced Fatty Liver Rats
Publikationsverlauf
received May 3, 2010
revised July 22, 2010
accepted July 26, 2010
Publikationsdatum:
17. August 2010 (online)
![](https://www.thieme-connect.de/media/plantamedica/201103/lookinside/thumbnails/10.1055-s-0030-1250268-1.jpg)
Abstract
The objectives of this study were to determine the effect of osthole on the insulin resistance (IR) in high-fat and high-sucrose-induced fatty liver rats and to investigate its potential mechanisms. The rat model was established by orally feeding high-fat and high-sucrose emulsion by gavage for 9 weeks. The experimental rats were treated with osthole 5 and 10 mg/kg, lipanthyl 30 mg/kg, and rosiglitazone 4 mg/kg after oral high-fat and high-sucrose emulsion for 6 weeks and were sacrificed 4 weeks after administration. The total cholesterol (TC), triglycerides (TG), and free fatty acids (FFA) in serum and hepatic tissue, fasting blood glucose (FBG), fasting serum insulin (FINS), serum adiponectin, and liver weight were measured. The homeostasis model assessment of insulin resistance (HOMA‐IR) and coefficient of hepatic weight were calculated. The results showed that after treatment with osthole, the serum levels of TC, TG, and FFA, the contents of TG and FFA in hepatic tissue, and body weight gain were lowered, especially in the osthole 10 mg/kg group (p < 0.05 or p < 0.01). Moreover, the histological evaluation of liver specimens demonstrated that the steatosis and inflammation in liver in osthole-treated groups were improved, especially in the 10 mg/kg group (p < 0.05). Importantly, the levels of FBG, FINS, and HOMA‐IR in the osthole 10 mg/kg group were decreased (p < 0.01), while the level of serum adiponectin in the osthole-treated groups, like PPARα agonist lipanthyl and PPARγ agonist rosiglitazone, was increased (p < 0.05). These results revealed that osthole could improve the IR induced by high-fat and high-sucrose emulsion in fatty liver rats, and its mechanism might be associated with increment of adiponectin release via activation of PPARα/γ pathway.
Key words
osthole - Cnidium monnieri - Apiaceae - insulin resistance - fatty liver - PPARα/γ - adiponectin
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Dr. Meilin Xie
Department of Pharmacology
Medical College of Soochow University
199 Renai Road, Suzhou Industrial Park
Suzhou 215123
Jiangsu Province
China
Telefon: + 86 5 12 69 56 65 53
Fax: + 86 5 12 65 88 20 89
eMail: xiemeilin@suda.edu.cn