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Cent Eur Neurosurg 2010; 71(4): 173-180
DOI: 10.1055/s-0030-1249698
Original Article

© Georg Thieme Verlag KG Stuttgart · New York

Expression of Invasion-Related Extracellular Matrix Molecules in Human Glioblastoma Versus Intracerebral Lung Adenocarcinoma Metastasis

I. Varga1 , G. Hutóczki2 , M. Petrás3 , B. Scholtz4 , E. Mikó4 , A. Kenyeres5 , J. Tóth6 , G. Zahuczky4 , L. Bognár2 , Z. Hanzély7 , A. Klekner2
  • 1Kenezy Hospital, Debrecen, Department of Pulmonology, Debrecen, Hungary
  • 2University of Debrecen, Department of Neurosurgery, Debrecen, Hungary
  • 3Medical and Health Science Center, University of Debrecen, Biophysics and Cell Biology, Debrecen, Hungary
  • 4University of Debrecen, Department of Biochemistry and Molecular Biology, Debrecen, Hungary
  • 5University of Debrecen, Department of Anatomy, Histology and Embryology, Debrecen, Hungary
  • 6University of Debrecen, Department of Oncology, Debrecen, Hungary
  • 7National Institute of Neurosurgery, Department of Pathology, Budapest, Hungary
Further Information

Publication History

Publication Date:
15 April 2010 (online)

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Abstract

Tumor cell invasion into the surrounding brain tissue is mainly responsible for the failure of radical surgical resection, with tumor recurrence in the form of microdisseminated disease. Extracellular matrix (ECM)-related molecules and their receptors predominantly participate in the invasion process, including cell adhesion to the surrounding microenvironment and cell migration. The extent of infiltration of the healthy brain by malignant tumors strongly depends on the tumor cell type. Malignant gliomas show much more intensive peritumoral invasion than do metastatic tumors. In this study, the mRNA expression of 30 invasion-related molecules (twenty-one ECM components, two related receptors, and seven ECM-related enzymes) was investigated by quantitative reverse transcriptase-polymerase chain reaction. Fresh frozen human tissue samples from glioblastoma (GBM), intracerebral lung adenocarcinoma metastasis, and normal brain were evaluated. Significant differences were established for 24 of the 30 molecules. To confirm our results at the protein level, immunohistochemical analysis of seven molecules was performed (agrin, neurocan, syndecan, versican, matrix metalloproteinase 2 [MMP-2], MMP-9, and hyaluronan). Determining the differences in the levels of invasion-related molecules for tumors of different origins can help to identify the exact molecular mechanisms that facilitate peritumoral infiltration by glioblastoma cells. These results should allow the selection of target molecules for potential chemotherapeutic agents directed against highly invasive malignant gliomas.

Key words

glioblastoma - metastasis - lung cancer - tumor invasion - extracellular matrix

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Correspondence

Dr. A. Klekner

University of Debrecen MHSC Hungary

Department of Neurosurgery

4030 Debrecen

Nagyerdei krt 98

4032 Debrecen

Hungary

Phone: +36/52/419 418

Fax: +36/52/419 418

Email: aklekner@yahoo.com