J Knee Surg 2009; 22(3): 196-204
DOI: 10.1055/s-0030-1247749
Original Article

© 2009 Thieme Medical Publishers

Fixation of Tissue-Engineered Human Neocartilage Constructs with Human Fibrin in a Caprine Model

Paul B. Lewis1 , L. Pearce McCarty4 , Jian Q. Yao5 , James M. Williams2 , Richard Kang1 , Brian J. Cole3
  • 1The Department of Orthopaedic Surgery, Rush University Medical Center, Chicago, Ill
  • 2The Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago, Ill
  • 3The Department of Orthopedics, Department of Orthopaedic Surgery, and the Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago, Ill
  • 4Sports and Orthopaedic Specialists, Minneapolis, Minn
  • 5Cartilage and Soft Tissue Biologics R&D, Zimmer Inc, Austin, Tex
Further Information

Publication History

Publication Date:
14 January 2010 (online)

ABSTRACT

The primary objective of this study was to determine the retention rate of neocartilage constructs in caprine full-thickness cartilage defects by fibrin sealant alone. Two defects, one each on the trochlea and the medial femoral condyle, were created in the stifle joint in 12 goats. Eight goats (16 defects) were treated with neocartilage constructs and 4 goats (8 defects) with fibrin glue alone. Postoperative activity was protected weight bearing for 6 weeks and then unrestricted for 18 weeks. At 24-week procurement, 4 neocartilage constructs were retained in 8 medial femoral defects and 4 in 8 trochlear defects. In gross comparison, the defects that retained the construct had a mean grade significantly higher than defects treated with fibrin glue alone. The mean histological score of defects with retained constructs was also higher than those treated with fibrin sealant alone. There was no appreciable immunologic reaction to the human neocartilage xenograft or human fibrin sealant.