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DOI: 10.1055/s-0029-1234765
Chemical constituents and antibacterial activity against bacteria causing foot odor of three extraction methods from the pummelo peel oil
The flavedo from fresh peels of Pummelo (Citrus maxima (Burm) Merr.) [1] were extracted by three different extraction methods; hydrodistillation [2], steam distillation and hexane extraction. The total yields from hydrodistillation, steam distillation and solvent extraction were 2.25, 1.83 and 1.47% w/w, respectively. Each oil samples were analyzed for chemical components by gas chromatograph and mass spectrometer (GC-MS). The highest content of monoterpene hydrocarbons i.e., limonene, phellandrene, α-pinene, were found in the extracts obtained from hydrodistillation (95.12%, 0.65%, and 0.61%, respectively). They were also investigated for their antimicrobial activities against bacterial causing foot odor, including Bacillus subtilis (ATCC 6633), Staphylococcus aureus (ATCC 6538), and Staphylococcus epidermidis (ATCC 12228) [3], using broth microdilution method [4]. The minimum inhibitory concentration (MIC) of the oil obtained from hydrodistillation with the most potency is 0.125, 0.125 and 0.03125% v/v for Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis, respectively.
References: [1] Smitinand, T. (2001) Thai plant names. The forest research office. Bangkok.
[2] Atti-Santos, A.C. et al. (2005)J. Braz. Arch. Biol. Techn. 48:155–160.
[3] Katsutoshi, A. et al. (2006) Can. J. Microbiol. 52:357–364.
[4] Ferraro, M.J. et al. (2000) Methods of dilution antimicrobial susceptibility test for bacteria that grow aerobically; approved standard-fifth. NCCLS. USA.