Planta Med 2009; 75 - PD82
DOI: 10.1055/s-0029-1234561

HPLC determination of certain flavonoids in Ginkgo biloba L.

A Ibrulj 1, E Sofic 2, I Tahirovic 2, E Kovac-Besovic 3
  • 1Institute for Quality Control of Medicines, M. Tita 9, 71000 Sarajevo, Bosnia and Herzegovina
  • 2Faculty of Science, University of Sarajevo, Zmaja od Bosne 33, 71000 Sarajevo, Bosnia and Herzegovina
  • 3Faculty of Pharmacy, University of Sarajevo, Cekalusa 90, 71000 Sarajevo, Bosnia and Herzegovina

Ginkgo, Ginkgo biloba L. (Ginkgoaceae, Ginkgoales), is probably the oldest living tree one earth (190–200 million years) and is commonly referred to as a living fossil.

The herbal material was gathered from two different locations in Sarajevo in the period from April until November 2008 (location A – Park at City centre and Location B – Botanical Gardens at National Museum of Bosnia and Herzegovina). It was air-dried and pulverized in a grinder for drugs just before the analysis.

This study investigated the flavonoids, which include three main aglycone (isorhamnetin, kaempferol, quercetin) derivates. Flavonoids are the major active constituents in G. biloba. The specific concentrations of these substances in the leaves vary by season. The aim of this work was separation and assay flavonoids by HPLC method of Ginkgo biloba extracts. Reversed-phase liquid chromatography method with ODS column, and mobile phases 0.3% H3PO4 pH 2.0 (A solvent) and methanol (B solvent) were recommended to separate this kind of substances. At a flow rate of 1ml/min, a column temperature 30 oC and UV detector was set at λ=370nm. At the location A in period from April until November the highest content of flavonoids was 0.50%, and at location B, in the same period, it was 0.17%. Conclusion: HPLC method, carried out for determination of various flavonoids in Ginkgo biloba, is very precise and suitable.

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2. European Pharmacopoeia, 6thed. Monograph 01/2008:1828.