Planta Med 2009; 75 - PD49
DOI: 10.1055/s-0029-1234528

Primary preventive effects of Kinginka tea on metabolic syndrome (Part 2)

H Oku 1, Y Ogawa 2, E Iwaoka 3, Y Yamaguchi 4, M Kunitomo 1, K Ishiguro 1
  • 1School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University, Koshien Kyuban-cho Nishinomiya, 663–8179, Japan
  • 2Faculty of Pharmaceutical Sciences, Doshisha Women's University, Kodo Kyotanabe Kyoto, 610–0395, Japan
  • 3Department of Pharmacy, Hyogo University of Health Sciences, Minatoshima Chuou-ku Kobe, 650–8530, Japan
  • 4Kaken-shoyaku Co., Ltd., Asahi-ku, Osaka, 535–0005, Japan

Metabolic syndrome, which has been increasing rapidly, complicates lifestyle related disease such as obesity, hypertension, hyperlipidemia and diabetes. We previously developed an in vivo assay method to search for primary preventive substances of the metabolic syndrome, monitors the decrease of peripheral blood flow due to the onset of the metabolic syndrome in SHR/NDmcr-cp/cp (SHR/cp) rats of a model [1]. Using this method, we previously found that Kinginka tea (the buds of Lonicera japonica L.) may reduce the risk factors of metabolic syndrome by preventing and improving the circulatory system (peripheral blood flow and blood pressure) if consumed daily [1]. In this study, we reported active mechanisms and compounds of Kinginka tea.

Kinginka tea significantly inhibited elevated serum level of lipid peroxide (LPO) and 8-hydroxydeoxyguanosine (8-OHdG), such as oxidative stress markers, 3-nitrotyrosine (3-NT) and 3-chlorotyrosyne (3-Cl), such as inflammatory markers in SHR/cp rats. The increase of 3-NT and 3-Cl are caused by the activation of the macrophage and neutrophilic leukocyte by the oxidation stress. Thus, one active mechanism of Kinginka tea may be preventive effects on vascular damage induced oxidative stress. Furthermore, by bioassay-directed fractionation of Kinginka tea, chlorogenic acid (1), luteolin (2), luteolin 7-glucoside (3), loganin (4), swerside (5) and secoxyloganin (6) were isolated. Compound 1, a major constituent of this tea significantly improved the decrease of peripheral blood flow in SHR/cp rats. Some various bioactivities of compound 1 on lifestyle-related disease, such as antioxidant action [2,3] have been reported. However, to our knowledge, this is the first report of the primary preventive activity of compound 1 on metabolic syndrome using SHR/cp rats.

References: [1] Oku, H. et al. (2007) Clin. Exp. Pharmacol. Physiol. 34:S40–42.

[2] Sakamoto, W. et al. (2003) Toxicology 183:255–263.

[3] Nakajima, Y. et al. (2007) Life Sci. 80:370–377.